2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. nAChR
  4. AChRα(97-116)

AChRα(97-116) is a synthetic peptide corresponding to the 97-116 region of the rat acetylcholine receptor α-subunit, and also an inducer of autoimmune diseases. AChRα(97-116) can be used in studies related to experimental autoimmune myasthenia gravis.

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AChRα(97-116)

AChRα(97-116) Chemical Structure

CAS No. : 663154-30-1

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Based on 1 publication(s) in Google Scholar

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AChRα(97-116) Related Antibodies

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Description

AChRα(97-116) is a synthetic peptide corresponding to the 97-116 region of the rat acetylcholine receptor α-subunit, and also an inducer of autoimmune diseases. AChRα(97-116) can be used in studies related to experimental autoimmune myasthenia gravis[1][2][3].

In Vitro

AChRα(97-116) (10 μg/mL; 72 h) does not induce significant proliferation of lymphocytes harvested from MBP68-86-immunized Lewis rats[1].
AChRα(97-116) (10 μg/mL; 48 h) maintains the antigen-specific immune cell phenotype responsive to dopaminergic modulation in recall-stimulated EAMG rat-derived CD4+ T cells and B220+ B cells[2].
AChRα(97-116) (2 μg/mL; overnight at 4 °C) effectively detects elevated AChR-specific antibody levels in EAMG rat serum and dopamine-stimulated EAMG rat splenocyte supernatants[2].
AChRα(97-116) (10 μg/mL; 24 h pre-incubation + 24 h co-culture) stimulates CD4+ T cells from EAMG rats to differentiate into AChR-specific Tfh cells at a 3-4-fold higher ratio than in control rats, and these Tfh cells promote increased IgG2b-secreting B cell percentages and anti-AChR antibody production in co-cultures with EAMG-derived B cells[3].
AChRα(97-116) (10 μg/mL; 72 h) stimulates lymph node-derived mononuclear cells from EAMG rats to produce elevated percentages of CD4+CXCR5+ and CD4+ICOS+ pre-Tfh cells following 72 h of incubation[3].
AChRα(97-116) (2 μg/mL; overnight at 4°C) coated on ELISA plates enables detection of significantly elevated anti-AChR antibody levels in serum from EAMG rats across multiple time points and dilutions compared to control rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AChRα(97-116) Related Antibodies
In Vivo

Immunization with AChRα(97-116) (50 μg/rat; tail root injection; two doses (first with Mycobacterium tuberculosis-containing emulsion, second on day 30 without Mycobacterium tuberculosis)) induces experimental autoimmune myasthenia gravis in female Lewis rats, characterized by increased clinical scores, reduced body weight, impaired neuromuscular junctions, and elevated AChR-specific antibodies[2].
Immunization with AChRα(97-116) (50 μg; subcutaneous injection; day 0 and day 30) induces experimental autoimmune myasthenia gravis in Lewis rats with >85% efficiency, driving elevated anti-AChR antibody production, reduced muscle AChR clusters, and expansion of AChR-specific pre-Tfh and mature Tfh cells that promote germinal center formation and B cell antibody secretion[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Lewis rats (female, 6-8 weeks old, 140-160 g, immunization-induced experimental autoimmune myasthenia gravis)[2]
Dosage: 50 μg/rat
Administration: tail root injection; two doses (first with Mycobacterium tuberculosis-containing emulsion, second on day 30 without Mycobacterium tuberculosis)
Result: Induced a significant increase in clinical scores from day 40 onward.
Caused a significant decline in body weight starting on day 42 after the second immunization.
Reduced α-bungarotoxin fluorescence intensity and caused intermittent morphology at neuromuscular junctions.
Elevated serum AChR-specific antibody levels significantly.
Molecular Weight

2252.56

Formula

C106H162N24O30
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Asp-Gly-Asp-Phe-Ala-Ile-Val-Lys-Phe-Thr-Lys-Val-Leu-Leu-Asp-Tyr-Thr-Gly-His-Ile
Sequence Shortening

DGDFAIVKFTKVLLDYTGHI
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

0.5 M NaOH : 100 mg/mL (44.39 mM; Need ultrasonic)

DMSO : 28.57 mg/mL (12.68 mM; ultrasonic and adjust pH to 2 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4439 mL 2.2197 mL 4.4394 mL
5 mM 0.0888 mL 0.4439 mL 0.8879 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.22 mg/mL (0.99 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.22 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.2 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.22 mg/mL (0.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.22 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (22.2 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.41%

SDS (252 KB)

COA (245 KB) LCMS (103 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / 0.5 M NaOH 1 mM 0.4439 mL 2.2197 mL 4.4394 mL 11.0985 mL
5 mM 0.0888 mL 0.4439 mL 0.8879 mL 2.2197 mL
10 mM 0.0444 mL 0.2220 mL 0.4439 mL 1.1098 mL
0.5 M NaOH 15 mM 0.0296 mL 0.1480 mL 0.2960 mL 0.7399 mL
20 mM 0.0222 mL 0.1110 mL 0.2220 mL 0.5549 mL
25 mM 0.0178 mL 0.0888 mL 0.1776 mL 0.4439 mL
30 mM 0.0148 mL 0.0740 mL 0.1480 mL 0.3699 mL
40 mM 0.0111 mL 0.0555 mL 0.1110 mL 0.2775 mL
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AChRα(97-116) Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AChRα(97-116)663154-30-1nAChRNicotinic acetylcholine receptorslymph node germinal centerpre-follicular helper T cellsforelimb muscle AChR clustersrat acetylcholine receptor α subunitmature follicular helper T cellsLewis ratsCD4+ T cellsexperimental autoimmune myasthenia gravisratsanti-AChR antibodyInhibitorinhibitorinhibit

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