Acyl Carrier Protein (ACP) (65-74) 

Acyl Carrier Protein (ACP) (65-74) is a key coenzyme fragment of fatty acid synthase (FAS) and participates in fatty acid synthesis as an acyl carrier. Acyl Carrier Protein (ACP) (65-74) mediates condensation, reduction and other cyclic reactions in fatty acid synthesis by covalently binding to acyl chains to form acyl-ACP intermediates, promoting acyl chain extension. Acyl Carrier Protein (ACP) (65-74) can regulate the synthesis ratio of saturated and unsaturated fatty acids. Acyl Carrier Protein (ACP) (65-74) can be used to study the mechanism of bacterial fatty acid synthesis and is a potential target for antibiotics (such as inhibiting the FAS system of pathogens)^[1][2][3].

1. Enzyme activity assay:
Acyl Carrier Protein (ACP) (65-74) (10 μM; 30 min) significantly enhances the catalytic efficiency of β-ketoacyl-ACP reductase (FabG) in in vitro enzymatic reactions, increasing the rate of NADPH-dependent reduction reactions by 3 times^[1].
2. Protein interaction assay:
Acyl Carrier Protein (ACP) (65-74) (5 μM; 2 h) specifically binds to β-ketoacyl-ACP synthase III (FabH) in surface plasmon resonance (SPR) experiments with a dissociation constant K of 2.3 μM. The two may interact through a conserved acidic helical region^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1063.16

C₄₇H₇₄N₁₂O₁₆

66851-75-0

Solid

White to off-white

Val-Gln-Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly

VQAAIDYING

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture and light, under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Peng X, et al. An Acyl Carrier Protein Gene Affects Fatty Acid Synthesis and Growth of Hermetia illucens. Insects. 2023 Mar 21;14(3):300.  [Content Brief]

  [2]. Seo DW, et al. An endogenous proteinacious inhibitor in porcine liver for S-adenosyl-L-methionine dependent methylation reactions: identification as oligosaccharide-linked acyl carrier protein. Int J Biochem Cell Biol. 2000 Apr;32(4):455-64.  [Content Brief]

  [3]. Chan DI, et al. Current understanding of fatty acid biosynthesis and the acyl carrier protein. Biochem J. 2010 Aug 15;430(1):1-19.  [Content Brief]