Adenosine phosphonobutyric, 2'(3'), 5'-diposphate  (Synonyms: APBADP)

Adenosine phosphonobutyric, 2'(3'), 5'-diposphate (APBADP) is an adenylosuccinate lyase substrate analog and competitive inhibitor. Adenosine phosphonobutyric, 2'(3'), 5'-diposphate binds to the tetramers of *Bacillus subtilis* adenylosuccinate lyase and wild-type human adenylosuccinate lyase. Adenosine phosphonobutyric, 2'(3'), 5'-diposphate can be used for research on adenylosuccinate lyase deficiency^[1].

adenylosuccinate lyase (ASL)^[1]

Adenosine phosphonobutyric, 2'(3'), 5'-diphosphate (0-1 μM; 30 min) acts as a competitive inhibitor of wild-type Bacillus subtilis adenylosuccinate lyase, targeting the substrates SAMP and SAICAR with K_i values of 0.18 μM and 0.16 μM, respectively^[1].
APBADP (0.25-1.0 μM; 30 min) acts as a competitive inhibitor of wild-type human adenylosuccinate lyase against the substrates SAMP and SAICAR, with K_i values of 0.09 μM and 0.21 μM, respectively^[1].
APBADP (30 μM) binds to mutant adenylosuccinate lyase variants of *Bacillus subtilis* (H141Q, H68A, H89Q, H89R, D69N), with a binding level comparable to that of the wild-type enzyme^[1].
APBADP (30 μM) binds to serine-mutated variants of *Bacillus subtilis* and *Homo sapiens* adenylosuccinate lyase^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

609.27

C₁₄H₂₂N₅O₁₆P₃

1050202-63-5

O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(O)=O)O[C@H]1N2C(N=CN=C3NC(C(O)=O)CCOP(O)(O)=O)=C3N=C2

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• [1]. Sivendran S, et al. Effect of a new non-cleavable substrate analog on wild-type and serine mutants in the signature sequence of adenylosuccinate lyase of Bacillus subtilis and Homo sapiens. Protein Sci. 2008;17(7):1162-1174.  [Content Brief]