Allylthiourea (Synonyms: Thiosinamine; N-Allylthiourea)

製品番号: HY-B0543 純度: 99.95%: Data Sheet SDS COA 取扱説明書
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Allylthiourea can selectively inhibit the oxidation of ammonia. Allylthiourea is commonly used to inhibit nitrification by targeting ammonia monooxygenase and chelating copper in the active site to suppress its activity. Allylthiourea also exhibits anticancer activity, showing cytotoxicity against the MCF-7 cell line with an IC₅₀ of 5.22 mM. Allylthiourea can be utilized in research related to micropollutant biodegradability and cancer studies.

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Allylthiourea 構造式

CAS 番号 : 109-57-9

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 38	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 38	在庫あり	Estimated Time of Arrival: December 31
Solid
25 g	$35	在庫あり	Estimated Time of Arrival: December 31
50 g	$55	在庫あり	Estimated Time of Arrival: December 31
100 g	$85	在庫あり	Estimated Time of Arrival: December 31
500 g	$170	在庫あり	Estimated Time of Arrival: December 31
> 100 g		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

Other Forms of Allylthiourea:

Allylthiourea (Standard) お問い合わせ

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•関連タンパク質:

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

体外実験

Allylthiourea (10 μM, 10 min) selectively inhibits ammonia oxidizers in vitro^[1].
Allylthiourea (15 μM, 70 h) decreases intracellular copper in Methylosinus trichosporium OB3b, allowing sMMO production at Cu/biomass ratios normally not permitting sMMO synthesis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

116.18

分子式

C₄H₈N₂S

CAS 番号

109-57-9

Appearance

Solid

Color

White to light yellow

SMILES

S=C(N)NCC=C

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : ≥ 100 mg/mL (860.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (430.37 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.6073 mL	43.0367 mL	86.0733 mL
5 mM	1.7215 mL	8.6073 mL	17.2147 mL
10 mM	0.8607 mL	4.3037 mL	8.6073 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (21.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.95%

Select Batch:

データシート (270 KB) SDS (479 KB)

COA (243 KB) HNMR (239 KB) LCMS (96 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Ginestet, P., et al., Estimation of nitrifying bacterial activities by measuring oxygen uptake in the presence of the metabolic inhibitors allylthiourea and azide. Appl Environ Microbiol, 1998. 64(6): p. 2266-8. [Content Brief]

[2]. Yu, Y., J.A. Ramsay, and B.A. Ramsay, Use of allylthiourea to produce soluble methane monooxygenase in the presence of copper. Appl Microbiol Biotechnol, 2009. 82(2): p. 333-9. [Content Brief]

[3]. Tatari K, et al. Challenges in using allylthiourea and chlorate as specific nitrification inhibitors[J]. Chemosphere, 2017, 182: 301-305. [Content Brief]

[4]. Widiandani T, et al. New N-allylthiourea derivatives: synthesis, molecular docking and in vitro cytotoxicity studies[J]. Tropical Journal of Pharmaceutical Research, 2018, 17(8): 1607-1613.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.6073 mL	43.0367 mL	86.0733 mL	215.1833 mL
	5 mM	1.7215 mL	8.6073 mL	17.2147 mL	43.0367 mL
	10 mM	0.8607 mL	4.3037 mL	8.6073 mL	21.5183 mL
	15 mM	0.5738 mL	2.8691 mL	5.7382 mL	14.3456 mL
	20 mM	0.4304 mL	2.1518 mL	4.3037 mL	10.7592 mL
	25 mM	0.3443 mL	1.7215 mL	3.4429 mL	8.6073 mL
	30 mM	0.2869 mL	1.4346 mL	2.8691 mL	7.1728 mL
	40 mM	0.2152 mL	1.0759 mL	2.1518 mL	5.3796 mL
	50 mM	0.1721 mL	0.8607 mL	1.7215 mL	4.3037 mL
	60 mM	0.1435 mL	0.7173 mL	1.4346 mL	3.5864 mL
	80 mM	0.1076 mL	0.5380 mL	1.0759 mL	2.6898 mL
	100 mM	0.0861 mL	0.4304 mL	0.8607 mL	2.1518 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Allylthiourea Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Allylthiourea109-57-9Thiosinamine N-AllylthioureaReactive Oxygen Species (ROS)oxidation of ammonianitrificationMCF-7 cell linemicropollutant biodegradabilityInhibitorinhibitorinhibit

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