Anti-Mouse TIM-4 Antibody (RMT4-53) 

Anti-Mouse TIM-4 Antibody (RMT4-53) is a rat-derived IgG2b κ type antibody inhibitor, targeting to mouse TIM-4. Anti-Mouse TIM-4 Antibody (RMT4-53) can block T cell immunoglobulin and mucin domain 4 (TIM-4). Anti-Mouse TIM-4 Antibody (RMT4-53) can inhibit the engulfment of apoptotic cells and T cell proliferation. Anti-Mouse TIM-4 Antibody (RMT4-53) can be used for the researches of cancer, immunology and metabolic disease, such as MC38 tumor and graft^[1][2][3].

Rat IgG2b kappa

Rat IgG2b kappa, Isotype Control

Mouse

TIM-4

Anti-Mouse TIM-4 Antibody (RMT4-53) (2-4 h) inhibits T cell STAT6 activation in CD4⁺ T cells ^[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (4 h) inhibits phagocytosis of apoptotic EG7 cells in Timd4^−/− and WT peritoneal macrophages^[2].
Anti-Mouse TIM-4 Antibody (RMT4-53) (4 h) augments cross-priming of OVA-specific T cells in TIM-4⁺ bone-marrow derived macrophages (BMDMs)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse TIM-4 Antibody (RMT4-53) (500 μg at days 0 and 250 μg at days 2, 4 and 6, i.p.) reduces numbers of allospecific Thy1.2⁺ T cells in both spleen and draining lymph nodes in ABMtg GFP^- CD4⁺ tg T cells transferred B6 Thy1.1⁺ mice of a bm12 skin graft^[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (500 μg at days 0 and 250 μg at days 2, 4, 6 and 8, i.p.) prolongs graft survival in B6 CD4⁺25^- T cells transferred B6 RAG^−/− mice of a bm12 skin transplant^[1].
Anti-Mouse TIM-4 Antibody (RMT4-53) (i.p., at days 8, 10 and 12) inhibits tumor growth companied with CDDP (HY-17394) in MC38 tumor Atg5^flox/flox mice^[2].
Anti-Mouse TIM-4 Antibody (RMT4-53) (0.5 mg/mouse, i.v., 1 h prior to ischemia) suppresses liver damage in segmental (70%) hepatic ischemia-reperfusion injury (IRI) mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

276891  [NCBI]

Q6U7R4 / NP_848874.3

in vivo TIM-4 blockade; in vitro TIM-4 blockade; Immunofluorescence

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse TIM-4 Antibody (RMT4-53)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yeung MY, et al. Interruption of dendritic cell-mediated TIM-4 signaling induces regulatory T cells and promotes skin allograft survival. J Immunol. 2013 Oct 15;191(8):4447-55.  [Content Brief]

  [2]. Baghdadi M, et al. TIM-4 glycoprotein-mediated degradation of dying tumor cells by autophagy leads to reduced antigen presentation and increased immune tolerance. Immunity. 2013 Dec 12;39(6):1070-81.  [Content Brief]

  [3]. Ji H, et al. T-cell immunoglobulin and mucin domain 4 (TIM-4) signaling in innate immune-mediated liver ischemia-reperfusion injury. Hepatology. 2014 Dec;60(6):2052-2064.  [Content Brief]