3-Nitrotyrosine Antibody

Cat. No.: HY-P81216: Fiche technique COA
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User Guide for Antibodies
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3-Nitrotyrosine Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 3-Nitrotyrosine.

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Size	Stock	Quantité
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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3-Nitrotyrosine Antibody Recommandations en vedette:

1 Publications Citing Use of MCE 3-Nitrotyrosine Antibody

•Small. 2025 Jun 26:e2503033. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Description
Références

Description

3-Nitrotyrosine Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 3-Nitrotyrosine.

Host

Rabbit

Clonality

Polyclonal

Masse moléculaire

Predicted band size: 226.19

Species Reactivity

Species independent Predicted Reactivity: Mouse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Gene ID

Immunogen

KLH conjugated Nitrotyrosine

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
FC FC: Flow Cytometry	1μg/Test
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Pureté	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

mIHC

Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using 3-Nitrotyrosine antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81216, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung cancer tissue using 3-Nitrotyrosine antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81216, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

Function：3-Nitrotyrosine/BSA (HY-161580)：3-Nitrotyrosine/BSA is an antigen-adjuvant conjugate formed by the conjugation of 3-Nitrotyrosine with Bovine Serum Albumin (BSA). By conjugating the antigen with a protein adjuvant, the production of antigen-specific antibodies in vaccine models can be enhanced. The conjugate does not affect protein folding or disrupt major epitopes, and it enhances cross-presentation and the generation of antigen-specific T cells.

RRID

AB_3103104

Synonyms

NO tyrosine; nTyr; Nitrotyrosine; nitro-Tyrosine; nitro Tyrosine.

Documentation

Select Batch:

Fiche technique (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/lclc-97tm1-cell.html

3-Nitrotyrosine Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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3-Nitrotyrosine Antibody

3-Nitrotyrosine Antibody Recommandations en vedette:

1 Publications Citing Use of MCE 3-Nitrotyrosine Antibody

Product Detail

Verification Image

Background

Description

Références

3-Nitrotyrosine Antibody Related Classifications

Vos produits récemment consultés:

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