eIF4EBP3 Antibody (YA1080)

Cat. No.: HY-P81335: Fiche technique COA
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eIF4EBP3 Antibody (YA1080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP3.

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Size	Stock	Quantité
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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eIF4EBP3 Antibody (YA1080) Recommandations en vedette:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

eIF4EBP3 Antibody (YA1080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP3.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 11 kDa;

Observed band size: 11 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60516

Gene ID

8637 [NCBI]

Immunogen

Recombinant protein of human EIF4EBP3.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Transfected	Pureté	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from CHO-K1 (lane 1, 15 μg), CHO-K1(EGFP-EIF4EBP3) (lane 2, 15 μg) using 4E BP3 (3B6) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: the hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repression of translation. In contrast, the hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity). Inhibits EIF4E-mediated mRNA nuclear export (PubMed:22684010)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:The gene is expressed at the highest levels in skeletal muscle, heart, kidneys, and pancreas, but at very low levels in the brain and thymus.

Subunit：Interacts with EIF4E (PubMed:9593750). Interacts with RPA2 (in unphosphorylated form via N-terminus); the interaction enhances EIF4EBP3-mediated inhibition of EIF4E-mediated mRNA nuclear export (PubMed:22684010)

Database

Entrez Gene: 8637 Human

SwissProt: O60516

OMIM: 603483 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

4EBP3; 4E-BP3

Documentation

Select Batch:

Fiche technique (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

eIF4EBP3 Antibody (YA1080) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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eIF4EBP3 Antibody (YA1080)

eIF4EBP3 Antibody (YA1080) Recommandations en vedette:

Product Detail

Verification Image

Background

Description

eIF4EBP3 Antibody (YA1080) Related Classifications

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