8-OHdG (DNA/RNA Damage) Antibody

製品番号: HY-P81140: データシート SDS COA User Guide for Antibodies
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8-OHdG (DNA/RNA Damage) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 8-OHdG (DNA/RNA Damage).

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容量	価格（税別）	在庫状況	数量
10 μL	$95	在庫あり	Estimated Time of Arrival: December 31
50 μL	$250	在庫あり	Estimated Time of Arrival: December 31
100 μL	$390	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

IHC

: 8-OHdG (DNA/RNA Damage) Antibody purchased from MCE. Usage Cited in: Engineering. 2025 Oct 31.; 8-OHdG(1;300) immunofluorescence staining in the temporal cortical region of mouse brain tissue.

: 8-OHdG (DNA/RNA Damage) Antibody purchased from MCE. Usage Cited in: ACS Appl Mater Interfaces. 2025 Oct 1;17(39):54484-54495. [Abstract]; Representative immunofluorescence images of mouse lung tissue sections stained for the oxidative stress marker 8-OHdG.

: 8-OHdG (DNA/RNA Damage) Antibody purchased from MCE. Usage Cited in: Basic Res Cardiol. 2025 Sep 16. [Abstract]; 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunofluorescence with MFI in mouse 3-hit hearts;

: 8-OHdG (DNA/RNA Damage) Antibody purchased from MCE. Usage Cited in: J Ethnopharmacol. 2025 Dec 30:360:121126. [Abstract]; Representative immunohistochemical staining of 8-OHdG in mouse liver tissues.

: 8-OHdG (DNA/RNA Damage) Antibody purchased from MCE. Usage Cited in: Mol Oncol. 2025 Jul 13. [Abstract]; Representative images (left) and quantification (right) of 8-oxo-dG–positive nuclei (green) in MDA-MB231 cells after 24 h of the indicated treatments. Nuclei were counterstained with DAPI (blue). Quantification represents the percentage of 8-oxo-dG–positive cells, with a minimum of 200 nuclei counted per independent experiment.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

8-OHdG (DNA/RNA Damage) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to 8-OHdG (DNA/RNA Damage).

Host

Rabbit

Clonality

Polyclonal

分子量

Predicted band size: 0.283kDa

Species Reactivity

Species independent

SwissProt ID

Gene ID

Immunogen

KLH conjugated 8-OHdG

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500
mIHC mIHC: Multiplex Immunohistochemical	1:500

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

mIHC

Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1：500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1：500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1：500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1：500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1：500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using 8-OHdG antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81140, 1：500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

Function：8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a marker formed in DNA after oxidation; guanine, the most sensitive base to free radical attack in DNA, can be converted to various markers such as 8-hydroxyguanine, 8-hydroxyguanosine, and 8-OHdG after oxidation. Among them, the 8-OHdG adduct is one of the most abundant base modifications excreted in urine, and due to its easy collection, it is commonly used as a biomarker for oxidative DNA damage. It is also the main product of DNA damage and can be used as an important biomarker to evaluate oxidative stress induced by exogenous compounds. Chronic arsenic exposure leads to elevated levels of urinary 8-OHdG, triclosan (TCS) exposure significantly increases 8-OHdG levels in zebrafish brain tissue, and levels of 8-OHdG in mitochondrial DNA isolated from the parietal cortex of patients with Alzheimer’s disease (AD) are significantly higher than those of age-matched controls. Additionally, the presence of 8-OHdG in DNA (also known as 8-hydroxyguanosine in RNA) is a reliable marker of base modifications, as it is consistently detected after oxidative stress generated by various conditions.

RRID

AB_3103069

Synonyms

8-hydroxy-2'-deoxyguanosine; 2'-Deoxy-8-oxoguanosine; 7,8-Dihydro-8-oxo-2'-deoxyguanosine; 8-Oxo-7,8-dihydro-2'-deoxyguanosine; 8-Oxo-7,8-dihydrodeoxyguanosine; 2'-Deoxy-8-oxo-D-guanosine; 8-Oxo-7,8-dihydro-2μ-deoxyguanosine,8-Oxo-dG; 2'-Deoxy-8-hydroxyguanosine;

ドキュメンテーション

Select Batch:

データシート (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/huo-3n1-cell.html