Aquaporin 5 Antibody

製品番号: HY-P81136: データシート SDS COA User Guide for Antibodies
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Aquaporin 5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 5.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量	価格（税別）	在庫状況	数量
20 μL	$135	在庫あり	Estimated Time of Arrival: December 31
50 μL	$200	在庫あり	Estimated Time of Arrival: December 31
100 μL	$325	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•J Ethnopharmacol. 2024 Dec 7:119207. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

Aquaporin 5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 5.

Host

Rabbit

Clonality

Polyclonal

分子量

Predicted band size: 29 kDa;

Observed band size: 27 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Rat

SwissProt ID

Q9WTY4

Gene ID

11830 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from mouse AQP5: 201-265/265

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Mouse Lung (lane 1) using Aquaporin 5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (By similarity). Plays an important role in fluid secretion in salivary glands (PubMed:10400615, PubMed:16571723, PubMed:18027168). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (PubMed:10619865, PubMed:12042359, PubMed:18027168)

Subcellular Localization：Apical cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Detected at the luminal membrane of secretory epithelial cells in hindpaw sweat glands (PubMed:12042359, PubMed:23473857) . Detected in acinar cells in salivary glands, in duct cells in lacrimal glands and in lung (at protein level) (PubMed:10337625, PubMed:10400615, PubMed:10619865, PubMed:16571723, PubMed:18027168) . Detected in lung, parotid, submandibular, sublingual, and lacrimal gland tissues (PubMed:10337625)

Subunit：Homotetramer; each monomer provides an independent water pore (By similarity). Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (PubMed:16571723)

RRID

AB_3103068

Database

Entrez Gene: 11830 Human ; 11830 Mouse ; 25241 Rat

SwissProt: Q9WTY4 Human ; Q9WTY4 Mouse ; P47864 Rat ;

Synonyms

Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/ht-3-cell.html