2. Antibodies
  3. Primary Antibodies
  4. Polyclonal Antibodies
  5. Aquaporin 5 Antibody

Aquaporin 5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 5.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Aquaporin 5 Antibody

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • Descripciòn

Descripciòn

Aquaporin 5 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Aquaporin 5.
Host

Rabbit
Clonality

Polyclonal
Peso molecular
Predicted band size: 29 kDa;
Observed band size: 27 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Mouse, Rat
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from mouse AQP5: 201-265/265
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
Sensitivity Endogenous Pureza affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol or 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Mouse Lung (lane 1) using Aquaporin 5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient (By similarity). Plays an important role in fluid secretion in salivary glands (PubMed:10400615, PubMed:16571723, PubMed:18027168). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Seems to play a redundant role in water transport in the eye, lung and in sweat glands (PubMed:10619865, PubMed:12042359, PubMed:18027168)
Subcellular Localization：Apical cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Multi-pass membrane protein
Expression：
Tissue_specificity:Detected at the luminal membrane of secretory epithelial cells in hindpaw sweat glands (PubMed:12042359, PubMed:23473857) . Detected in acinar cells in salivary glands, in duct cells in lacrimal glands and in lung (at protein level) (PubMed:10337625, PubMed:10400615, PubMed:10619865, PubMed:16571723, PubMed:18027168) . Detected in lung, parotid, submandibular, sublingual, and lacrimal gland tissues (PubMed:10337625)
Subunit：Homotetramer; each monomer provides an independent water pore (By similarity). Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (PubMed:16571723)
RRID
Database

Entrez Gene: 11830 Human ; 11830 Mouse ; 25241 Rat

SwissProt: Q9WTY4 Human ; Q9WTY4 Mouse ; P47864 Rat ;

Synonyms
Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.
Documentación
SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Aquaporin 5 Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
Aquaporin 5 Antibody
Cat. No.:
HY-P81136
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