Cleaved-Caspase 3 p12 Antibody (YA499)

製品番号: HY-P80623: データシート SDS COA User Guide for Antibodies
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Cleaved-Caspase 3 Antibody (YA499) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$70	在庫あり	Estimated Time of Arrival: December 31
50 μL	$185	在庫あり	Estimated Time of Arrival: December 31
100 μL	$300	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•Theriogenology. 2025 Mar 15:235:121-133.

IHC

: Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: Nat Metab. 2026 Mar 5.; Mediobasal hypothalamic sections (containing 3V tanycytes) from Rab27a KD and Con were immunostained for cleaved caspase 3.

: Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: Exp Eye Res. 2025 Jan 18:110244. [Abstract]; The western blot analysis demonstrated an elevated protein ratio of B-cell lymphoma 2 (Bcl-2) to Bax and an increased level of cleaved-Caspase-3 after AOH injury. Notably, these effects were significantly attenuated following TAT-N24 treatment.

: Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: Theriogenology. 2025 Mar 15:235:121-133.; The proapoptotic function of Caspase 3 usually relies on its activated protein forms, specifically cleaved Caspase 3 (CC3). CC3 protein levels were assessed in GC-2 cells treated with miR-143 inhibitor or mimics using WB.

: Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: J Transl Med. 2024 May 17;22(1):469. [Abstract]; lncRNA RP11-197K6.1 knockdown increased the levels of apoptosis-related proteins, including cleaved caspase 3, caspase 7, and cleaved caspase 9, thus suggesting the activation of intrinsic apoptotic pathways. Notably, no significant change was observed in the expression level of cleaved caspase 8.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

Cleaved-Caspase 3 Antibody (YA499) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 32 kDa;

Observed band size: 32, 12 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P42574

Gene ID

836 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Caspase-3 p12.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:It is highly expressed in the lungs, spleen, heart, liver, and kidneys. It is moderately expressed in the brain and skeletal muscle, and lowly expressed in the testes. It is also present in many cell lines, with the highest expression in immune system cells.

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce

RRID

AB_3669005

Database

Entrez Gene: 836 Human

SwissProt: P42574 Human

OMIM: 600636 Human

Research Field

Cell Biology

Synonyms

CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

ドキュメンテーション

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データシート (233 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)