Lamin B2 Antibody (YA989)

製品番号: HY-P81283: データシート SDS COA User Guide for Antibodies
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Lamin B2 Antibody (YA989) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lamin B2.

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容量	価格（税別）	在庫状況	数量
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

Lamin B2 Antibody (YA989) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lamin B2.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 70 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q03252

Gene ID

84823 [NCBI]

Immunogen

Recombinant human Lamin B2 protein.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), MDA-MB-231 (lane 2, 15 μg), Hela (lane 3, 15 μg), and U-87MG (lane 4, 15 μg) using Lamin B2 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane (PubMed:33033404). Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics (PubMed:33033404). The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively (PubMed:33033404)

Subcellular Localization：Nucleus lamina

Subunit：Dimer (By similarity). Lamin dimers then assemble into dimeric head-to-tail polymers (By similarity). Ultimately, two head-to-tail polymers assemble laterally into a protofilament with a uniformly shaped rod of 3.5 nm in diameter (By similarity). Interacts with TMEM43 (By similarity)

Database

Entrez Gene: 84823 Human ; 16907 Mouse ; 299625 Rat

SwissProt: Q03252 Human ; P21619 Mouse ;

OMIM: 608709 Human

Research Field

Cell Biology

Synonyms

LMN2; LAMB2

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/nci-h3122-cell.html [Content Brief]