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  5. LINE-1 ORF1p Antibody (YA3039)

LINE-1 ORF1p Antibody (YA3039)

Cat. No.: HY-P83294
COA
SDS
User Guide for Antibodies Technical Support

LINE-1 ORF1p Antibody (YA3039) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LINE-1 ORF1p.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

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Descripciòn

LINE-1 ORF1p Antibody (YA3039) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LINE-1 ORF1p.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Peso molecular
Predicted band size: 40 kDa;
Observed band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human LINE-1 ORF1p aa157-330.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:100
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), A549 (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using LINE 1 ORF1p Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded
Subcellular Localization：Nucleus, nucleolus; Cytoplasm; Cytoplasm, Cytoplasmic ribonucleoprotein granule; Cytoplasm, Stress granule
Subunit：Homotrimer (via coiled coil domain). May also form larger homooligomers. May interact with DDX39A, HNRNPA1, SERBP1 and YBX1. Interacts with TEX19 and UBR2 (PubMed:28806172). Interacts with MOV10 (PubMed:23093941). Interacts with APOBEC3D; this interaction inhibits LINE-1 retrotransposition (PubMed:27428332)
RRID
Database

Entrez Gene: 105370531 Human

SwissProt: Q9UN81 Human

OMIM: 151626 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
L1ORF1p; L1RE1; LRE1
Documentación
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
Referencias

LINE-1 ORF1p Antibody (YA3039) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
LINE-1 ORF1p Antibody (YA3039)
Cat. No.:
HY-P83294
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