2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Tag Antibodies
  5. Myc-tag Antibody (YA702)

Myc-tag Antibody (YA702) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Myc-tag.

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50 μL En stock
100 μL En stock
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Descripciòn

  • Referencias

Descripciòn

Myc-tag Antibody (YA702) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Myc-tag.
Host

Mouse
Clonality

Monoclonal
Peso molecular
Predicted band size: 50 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Species independent
Immunogen

Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:5000
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
Use at an assay dependent concentration.
IP
IP: Immunoprecipitation
2-5 µg/mL.
Sensitivity Endogenous Pureza Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Myc-tag protein BACT2(lane 2(5ng) , BACT2 (lane 3(10ng) , using Myc-tag Antibody (HY-P80232). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background
Function：The Myc tag is a short peptide tag derived from the c-Myc protein. It has no biological function itself, but it can be used for the detection, localization, purification, and interaction studies of target proteins.
RRID
Research Field

Tags & Cell Markers
Documentación
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
Referencias

Myc-tag Antibody (YA702) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
Myc-tag Antibody (YA702)
Cat. No.:
HY-P80232
Cantidad:
MCE Japan Authorized Agent:

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