PRPF31 Antibody (YA3338)

Cat. No.: HY-P83593: Data Sheet COA
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User Guide for Antibodies
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PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

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사이즈	재고	수량
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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PRPF31 Antibody (YA3338) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8WWY3

Gene ID

26121 [NCBI]

Immunogen

A synthetic peptide of human PRPF31 aa 380-393.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from U251MG (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C2C12 (lane 4, 15 μg), and PC-12 (lane 5, 15 μg) using PRPF31 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:20118938, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543)

Subcellular Localization：Nucleus; Nucleus speckle; Nucleus, Cajal body

Expression：
Tissue_specificity:Ubiquitously expressed

Isoforms & Post-Translational Modification：Q8WWY3 has 4 isomers: Q8WWY3-1: 55456 Da (predicted); Q8WWY3-2: 40779 Da (predicted); Q8WWY3-3: 30966 Da (predicted); Q8WWY3-4: 54909 Da (predicted).
Phosphorylated by PRP4K during spliceosome assembly

Subunit：Identified in the spliceosome B complex (PubMed:28781166). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (PubMed:11867543, PubMed:16723661, PubMed:26912367).

Database

Entrez Gene: 26121 Human ; 68988 Mouse ; 292536 Rat

SwissProt: Q8WWY3 Human ; Q8CCF0 Mouse ;

OMIM: 600138 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Protein 61K; hPrp31; U4/U6 snRNP 61 kDa protein

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

PRPF31 Antibody (YA3338) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PRPF31 Antibody (YA3338)

PRPF31 Antibody (YA3338) Featured Recommendations:

Product Detail

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Background

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PRPF31 Antibody (YA3338) Related Classifications

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