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  4. F4/80 Antibody (PE) (YA3452)

F4/80 Antibody (PE) (YA3452)

Art. -Nr.: HY-P83715M
COA User Guide for Antibodies Technical Support

F4/80 Antibody (YA3452) is a rat-derived and phycoerythrin (PE) IgG2b monoclonal antibody, targeting to F4/80.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Background

  • Beschreibung

Beschreibung

F4/80 Antibody (YA3452) is a rat-derived and phycoerythrin (PE) IgG2b monoclonal antibody, targeting to F4/80.

Host

Rat

Clonality

Monoclonal

Molekulargewicht
Predicted band size: 102 kDa;
Observed band size: 160 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Mouse
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from Mouse F4/80.

Application &
Dilution Ratio
Application Dilution Ratio
FC
FC: Flow Cytometry
5μL per test for million cells or whole blood in 100 μL volume.
Reinheit Affinity Purified Conjugation PE
Modification Unmodified Isotype IgG2b
Appearance

Liquid

Formulation

Supplied in PBS with 0.05% Proclin300, 1% BSA.

Storage & Stability

Stored at 2-8°C for 1 year, protect from light, do not freeze.

Versand

Shipping with blue ice.

Background
Function:Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development
Subcellular Localization:Cell membrane; Multi-pass membrane protein
Expression:
Tissue_specificity:It is expressed in macrophages; however, it is not expressed in macrophages in the T-cell regions of lymph nodes and spleen. Expression levels are low on blood monocytes.
RRID
Database

Entrez Gene: 13733 Mouse

SwissProt: Q61549 Mouse

Synonyms
Adhesion G protein-coupled receptor E1; Adgre1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; Adgre1; Emr1; Gpf480
Dokumentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Produktname:
F4/80 Antibody (PE) (YA3452)
Art. -Nr.:
HY-P83715M
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MCE Japan Authorized Agent: