T7 Tag Antibody (YA886)

Cat. No.: HY-P81040: Data Sheet COA
SDS
User Guide for Antibodies
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T7 Tag Antibody (YA886) is an unconjugated, mouse-derived, anti-T7 Tag (YA886) IgG1 monoclonal antibody.

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무료 샘플	Apply now
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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T7 Tag Antibody (YA886) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
제품 설명
References

제품 설명

T7 Tag Antibody (YA886) is an unconjugated, mouse-derived, anti-T7 Tag (YA886) IgG1 monoclonal antibody.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Species independent

Immunogen

Synthetic Peptide of T7-Tag

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Recombinant T7-Tag protein-10ng (lane 1, 10 ng), Recombinant T7-Tag protein-5ng (lane 2, 5 ng), Recombinant T7-Tag protein-2ng (lane 3, 2 ng), Recombinant T7-Tag protein-1ng (lane 4, 1 ng), Recombinant T7-Tag protein-0.5ng (lane 5, 0.5 ng), and Recombinant T7-Tag protein-0.1ng (lane 6, 0.1 ng) using T7 Tag Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Mouse IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：T7 Tag Peptide is a protein tag consisting of 11 residues from the N-terminus of the T7 bacteriophage capsid protein gp10. T7 Tag Peptide can be fused to the N-terminus or C-terminus of proteins such as enhanced green fluorescent protein (EGFP). T7 Tag Peptide can be used in various immunoassays and affinity purification.

RRID

AB_3103168

Research Field

Tags & Cell Markers

각종 서류

Select Batch:

Data Sheet (230 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/caov-3-cell-line-cell.html

T7 Tag Antibody (YA886) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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T7 Tag Antibody (YA886)

T7 Tag Antibody (YA886) Featured Recommendations:

Product Detail

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Background

제품 설명

References

T7 Tag Antibody (YA886) Related Classifications

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SAFETY DATA SHEET (SDS)

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