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  5. TIMP2 Antibody (YA034)

TIMP2 Antibody (YA034) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIMP2.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE TIMP2 Antibody (YA034)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • 제품 설명

  • References

제품 설명

TIMP2 Antibody (YA034) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TIMP2.
Host

Mouse
Clonality

Monoclonal
분자량
Predicted band size: 24 kDa;
Observed band size: 24 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

KLH conjugated synthetic peptide derived from human TIMP-2.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:20-100
FC
FC: Flow Cytometry
1:25
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG1  
Appearance

Liquid
Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from M-brain (lane 1) and M-lung (lane 2) using TIMP2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19
Subcellular Localization：Secreted
Expression：
Induction:Down-regulated by TGFB1
Subunit：Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity
RRID
Database

Entrez Gene: 7077 Human

SwissProt: P16035 Human

OMIM: 188825 Human

Research Field

Cardiovascular
Synonyms
Metalloproteinase inhibitor 2 precursor; Tissue inhibitor of metalloproteinases 2; Collagenase inhibitor; CSC 21K; CSC21K; TIMP2; TIMP 2; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP2_HUMAN; Metalloproteinase inhibitor 2; CSC-21K; TIMP-2.
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
References

TIMP2 Antibody (YA034) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
TIMP2 Antibody (YA034)
Cat. No.:
HY-P80356
수량:
MCE Japan Authorized Agent:

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SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

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