BODIPY-Cholesterol

Name: BODIPY-Cholesterol
Brand: MedChemExpress
SKU: HY-125746
Rating: 5.0 (14 reviews)

Cat. No.: HY-125746 Purity: 99.77%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 505/515 nm).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 878557-19-8

사이즈	재고	수량
1 mg	해외재고보유	Estimated Time of Arrival: December 31
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 14 publication(s) in Google Scholar

Other Forms of BODIPY-Cholesterol:

BODIPY-Cholesterol (solution) In-stock

14 Publications Citing Use of MCE BODIPY-Cholesterol

Cell Imaging/Staining

: BODIPY-Cholesterol purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May;21(5):934-956. [Abstract]; Representative confocal images of BODIPY-cholesterol (10 μg/mL; 24 h) staining after treatment of U2OS cells with indatraline (10 μM) or sertraline (10 μM) for 18 h were obtained.

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation^[1]
1.1 Prepare stock solution
Solvent: DMSO
Concentration: 1 mM is recommended (e.g., weigh 0.58 mg BODIPY-Cholesterol and dissolve in 1 mL solvent).
Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 0.5-5 μM (optimized according to the experiment) with PBS (or other buffer).
Note: The working solution should be prepared and used immediately, and protected from light.
2. Cell staining
2.1 Wash cells with PBS.
2.2 Label the cell suspension with BODIPY-Cholesterol (final concentration 0.5-5 μM), mix well and incubate at 37°C for 10-20 minutes.
2.3 Flow cytometric detection (Ex/Em = 505/515 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

576.61

화학식

C₃₆H₅₁BF₂N₂O

CAS No.

878557-19-8

Appearance

Solid

Color

Orange to reddish brown

Emission (Em)

515

Excitation (Ex)

505

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(C3=C(C)C=C(C)[N-]3[B+3]([F-])4[F-])=C5[N]4=C(C)C=C5C)([H])[C@@]6([H])[C@@](CC1)([H])[C@@]7(C(C[C@H](CC7)O)=CC6)C

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 1 mg/mL (1.73 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7343 mL	8.6714 mL	17.3427 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.77%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (251 KB) HNMR (305 KB) RP-HPLC (95 KB) MS (82 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: BODIPY-Cholesterol can be used as a fluorescent monitor for sterol uptake and inter-organelle sterol flux in cells.
Method: For cell staining.
1. Stain cells with BODIPY-Cholesterol (1 µM; 20 min; 37°C).
2. Use a microscope for observation.

References

[1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28. [Content Brief]

[2]. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO

1 mM

1.7343 mL

8.6714 mL

17.3427 mL

43.3569 mL