BODIPY-Cholesterol  (Synonyms: BODIPY-コレステロール)

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 505/515 nm)^[1].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation^[1]
1.1 Prepare stock solution
Solvent: DMSO
Concentration: 1 mM is recommended (e.g., weigh 0.58 mg BODIPY-Cholesterol and dissolve in 1 mL solvent).
Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 0.5-5 μM (optimized according to the experiment) with PBS (or other buffer).
Note: The working solution should be prepared and used immediately, and protected from light.
2. Cell staining
2.1 Wash cells with PBS.
2.2 Label the cell suspension with BODIPY-Cholesterol (final concentration 0.5-5 μM), mix well and incubate at 37°C for 10-20 minutes.
2.3 Flow cytometric detection (Ex/Em = 505/515 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

576.61

C₃₆H₅₁BF₂N₂O

515

505

878557-19-8

Solid

Orange to reddish brown

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(C3=C(C)C=C(C)[N-]3[B+3]([F-])4[F-])=C5[N]4=C(C)C=C5C)([H])[C@@]6([H])[C@@](CC1)([H])[C@@]7(C(C[C@H](CC7)O)=CC6)C

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28.  [Content Brief]

  [2]. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49.  [Content Brief]