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  4. BODIPY-Cholesterol

BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 505/515 nm).

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CAS. Nr. : 878557-19-8

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Based on 14 publication(s) in Google Scholar

Other Forms of BODIPY-Cholesterol:

BODIPY-Cholesterol Related Antibodies

Top Publications Citing Use of Products

    BODIPY-Cholesterol purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May;21(5):934-956.  [Abstract]

    Representative confocal images of BODIPY-cholesterol (10 μg/mL; 24 h) staining after treatment of U2OS cells with indatraline (10 μM) or sertraline (10 μM) for 18 h were obtained.

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    Beschreibung

    BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 505/515 nm)[1].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation[1]
    1.1 Prepare stock solution
    Solvent: DMSO
    Concentration: 1 mM is recommended (e.g., weigh 0.58 mg BODIPY-Cholesterol and dissolve in 1 mL solvent).
    Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 0.5-5 μM (optimized according to the experiment) with PBS (or other buffer).
    Note: The working solution should be prepared and used immediately, and protected from light.
    2. Cell staining
    2.1 Wash cells with PBS.
    2.2 Label the cell suspension with BODIPY-Cholesterol (final concentration 0.5-5 μM), mix well and incubate at 37°C for 10-20 minutes.
    2.3 Flow cytometric detection (Ex/Em = 505/515 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BODIPY-Cholesterol Related Antibodies
    Molekulargewicht

    576.61

    Formel

    C36H51BF2N2O
    CAS. Nr.
    Appearance

    Solid

    Color

    Orange to reddish brown

    Emission (Em)

    515
    Excitation (Ex)

    505
    SMILES

    C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(C3=C(C)C=C(C)[N-]3[B+3]([F-])4[F-])=C5[N]4=C(C)C=C5C)([H])[C@@]6([H])[C@@](CC1)([H])[C@@]7(C(C[C@H](CC7)O)=CC6)C
    Versand

    Room temperature in continental US; may vary elsewhere.
    Speicherung

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Lösungsmittel & Löslichkeit
    In Vitro: 

    DMSO : 1 mg/mL (1.73 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7343 mL 8.6714 mL 17.3427 mL
    5 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molaritätsrechner

    • Verdünnungsrechner

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Reinheit & Dokumentation

    Purity: 99.77%

    SDS (393 KB)

    COA (251 KB) HNMR (305 KB) RP-HPLC (95 KB) MS (82 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    Verweise

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7343 mL 8.6714 mL 17.3427 mL 43.3569 mL
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    BODIPY-Cholesterol Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BODIPY-Cholesterol878557-19-8Fluorescent DyeInhibitorinhibitorinhibit

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