1. Others Metabolic Enzyme/Protease
  2. Biochemical Assay Reagents Phosphatase
  3. Alkaline phosphatase substrate

Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays.

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Alkaline phosphatase substrate

Alkaline phosphatase substrate Chemische Struktur

CAS. Nr. : 160081-62-9

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1 mg (0.25 mM * 8.08 mL in Water) Auf Lager

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Beschreibung

Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays[1].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Mix the substance to be tested (phosphatase mimic) with the chemiluminescent substrate Alkaline phosphatase substrate (25 μM) in 20 mM HEPES buffer (pH 8.5).
2. Immediately measure the chemiluminescence intensity of the mixture at room temperature.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

495.20

Formel

C18H19Cl2Na2O7P

CAS. Nr.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

COC1(C(C=C2)=CC(O[P](O[Na])(O[Na])=O)=C2Cl)[C@]([C@H]3C4)(OO1)[C@@H](C[C@H]5C3)C[C@]4(C5)Cl

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Reinheit & Dokumentation

Purity: 95.30%

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  • Molaritätsrechner

  • Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)

Diese Gleichung wird häufig abgekürzt als: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Alkaline phosphatase substrate
Art. -Nr.:
HY-D1208
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