Alkaline phosphatase substrate 

Alkaline phosphatase substrate is a chemiluminescent substrate for alkaline phosphatase. Alkaline phosphatase substrate can be used for enzyme-linked immunoassays^[1].

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Mix the substance to be tested (phosphatase mimic) with the chemiluminescent substrate Alkaline phosphatase substrate (25 μM) in 20 mM HEPES buffer (pH 8.5).
2. Immediately measure the chemiluminescence intensity of the mixture at room temperature.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

495.20

C₁₈H₁₉Cl₂Na₂O₇P

160081-62-9

Liquid

Colorless to light yellow

COC1(C(C=C2)=CC(O[P](O[Na])(O[Na])=O)=C2Cl)[C@]([C@H]3C4)(OO1)[C@@H](C[C@H]5C3)C[C@]4(C5)Cl

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Hu L, et al. Chemiluminescence of the Ce(IV)/CDP-Star System Based on the Phosphatase-like Activity of Ce(IV) Ions. ACS Omega. 2021 Feb 25;6(9):6379-6384.  [Content Brief]