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  3. Fluorescent Dye
  4. CFDA-SE

CFDA-SE  (Synonyms: CFSE; 5(6)-Carboxyfluorescein diacetate succinimidyl ester; 5(6)-CFDA N-succinmidyl ester)

Cat. No.: HY-D0938 Pureza: 97.0%
COA
SDS
Instrucciones de manejo Technical Support

CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.

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No. CAS : 150347-59-4

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Revisión del cliente

Based on 107 publication(s) in Google Scholar

Other Forms of CFDA-SE:

CFDA-SE Related Antibodies

Top Publications Citing Use of Products

107 Publications Citing Use of MCE CFDA-SE

Cell Proliferation/Viability Assay
IF

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Aug 10:541:215746.  [Abstract]

    PBMCs are labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) (2.5 μM). The proliferation of T cells is assessed by CFSE intensity using flow cytometry.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2201899119.  [Abstract]

    Flow cytometry analysis of CD4+ T cell number (B), proliferation rate (C), and representative images of CFSE-based proliferation assay (D) of CD4+ T cells cocultured with AT2 cells from healthy, LLC-bearing GPX3fl/fl, or GPX3cKO mice respectively.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: OncoImmunology. 2022 Feb 1;11(1):2032918.  [Abstract]

    Bone marrow cells are incubated with 5 μΜ CFSE (5, 6- carboxyfluorescein diacetate) for 10 min at 37°C.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Feb 20;11(9):4251-4261.  [Abstract]

    Mouse MC38 cells are washed with PBS and labeled with 1 μM CFDA-SE in PBS for 30 minutes at room temperature.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Jul;25(14):7052-7065.  [Abstract]

    THP-1 cells are stained with 1 μM carbox fluorescenceindiacetate succinimidyl ester (CFSE) in 0.1% BSA for 10 minutes at 37°C and ended with PBS for 10 minutes.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Mol Nutr Food Res. 2022 Jan;66(2):e2100619.  [Abstract]

    CFDA-SE (10 μM) is added to bacterial suspension and then incubated at 37°C in the dark for 20 min. The four strains are labeled with fluorescent probe CFDA-SE and were gavaged to baby rats to evaluate their colonization ability. Compared to the control group, the CFDA-SE fluorescence is present in rats gavaged with the four strains.

    • Actividad biológica

    • Protocolo

    • Pureza y Documentación

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    • Revisión del cliente

    Descripciòn

    CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus[1]. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells[1].

    In Vitro

    Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
    Preparation of CFDA-SE working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg of CFDA-SE in 0.1794 mL of DMSO to obtain 10 mM of CFSE.
    Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of CFDA-SE working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of CFDA-SE working solution.
    Note: Please adjust the concentration of CFDA-SE working solution according to the actual situation.
    Cell staining
    2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    2.2 Add 1 mL of CFDA-SE working solution, and then incubate at room temperature for 30 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CFDA-SE Related Antibodies
    Peso molecular

    557.46

    Fòrmula

    C29H19NO11
    No. CAS
    Appearance

    Solid

    Color

    Off-white to yellow

    Emission (Em)

    515
    Excitation (Ex)

    485
    SMILES

    CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvente y solubilidad
    In Vitro: 

    DMSO : 50 mg/mL (89.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7939 mL 8.9693 mL 17.9385 mL
    5 mM 0.3588 mL 1.7939 mL 3.5877 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureza y Documentación

    Purity: 98.23%

    SDS (393 KB)

    COA (252 KB) HNMR (334 KB) LCMS (262 KB)

    Instrucciones de manejo (2659 KB)
    Dyeing Example
    Referencias
    Ensayo celular
    [2]

    Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].

    MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7939 mL 8.9693 mL 17.9385 mL 44.8463 mL
    5 mM 0.3588 mL 1.7939 mL 3.5877 mL 8.9693 mL
    10 mM 0.1794 mL 0.8969 mL 1.7939 mL 4.4846 mL
    15 mM 0.1196 mL 0.5980 mL 1.1959 mL 2.9898 mL
    20 mM 0.0897 mL 0.4485 mL 0.8969 mL 2.2423 mL
    25 mM 0.0718 mL 0.3588 mL 0.7175 mL 1.7939 mL
    30 mM 0.0598 mL 0.2990 mL 0.5980 mL 1.4949 mL
    40 mM 0.0448 mL 0.2242 mL 0.4485 mL 1.1212 mL
    50 mM 0.0359 mL 0.1794 mL 0.3588 mL 0.8969 mL
    60 mM 0.0299 mL 0.1495 mL 0.2990 mL 0.7474 mL
    80 mM 0.0224 mL 0.1121 mL 0.2242 mL 0.5606 mL
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    CFDA-SE Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CFDA-SE150347-59-4CFSE 5(6)-Carboxyfluorescein diacetate succinimidyl ester 5(6)-CFDA N-succinmidyl esterFluorescent DyeCell Proliferation Fluorescent ProbeInhibitorinhibitorinhibit

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    Nombre del producto:
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