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  3. Fluorescent Dye
  4. CFDA-SE

CFSE  (Synonyms: CFDA-SE; 5(6)-CFDA N-succinmidyl ester)

製品番号: HY-D0938 純度: 97.0%
COA 取扱説明書 Technical Support

CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.

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CAS 番号 : 150347-59-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
5 mg $50 在庫あり
10 mg $75 在庫あり
25 mg $160 在庫あり
50 mg $260 在庫あり
100 mg $380 在庫あり
200 mg $565 在庫あり
500 mg $905 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 107 publication(s) in Google Scholar

Other Forms of CFDA-SE:

CFSE 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Proliferation/Viability Assay
IF

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2022 Aug 10:541:215746.  [Abstract]

    PBMCs are labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) (2.5 μM). The proliferation of T cells is assessed by CFSE intensity using flow cytometry.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2022 Aug 9;119(32):e2201899119.  [Abstract]

    Flow cytometry analysis of CD4+ T cell number (B), proliferation rate (C), and representative images of CFSE-based proliferation assay (D) of CD4+ T cells cocultured with AT2 cells from healthy, LLC-bearing GPX3fl/fl, or GPX3cKO mice respectively.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: OncoImmunology. 2022 Feb 1;11(1):2032918.  [Abstract]

    Bone marrow cells are incubated with 5 μΜ CFSE (5, 6- carboxyfluorescein diacetate) for 10 min at 37°C.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Feb 20;11(9):4251-4261.  [Abstract]

    Mouse MC38 cells are washed with PBS and labeled with 1 μM CFDA-SE in PBS for 30 minutes at room temperature.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2021 Jul;25(14):7052-7065.  [Abstract]

    THP-1 cells are stained with 1 μM carbox fluorescenceindiacetate succinimidyl ester (CFSE) in 0.1% BSA for 10 minutes at 37°C and ended with PBS for 10 minutes.

    CFDA-SE purchased from MedChemExpress. Usage Cited in: Mol Nutr Food Res. 2022 Jan;66(2):e2100619.  [Abstract]

    CFDA-SE (10 μM) is added to bacterial suspension and then incubated at 37°C in the dark for 20 min. The four strains are labeled with fluorescent probe CFDA-SE and were gavaged to baby rats to evaluate their colonization ability. Compared to the control group, the CFDA-SE fluorescence is present in rats gavaged with the four strains.

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    製品説明

    CFDA-SE is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus[1]. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells[1].

    体外実験

    Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
    Preparation of CFDA-SE working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg of CFDA-SE in 0.1794 mL of DMSO to obtain 10 mM of CFSE.
    Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of CFDA-SE working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of CFDA-SE working solution.
    Note: Please adjust the concentration of CFDA-SE working solution according to the actual situation.
    Cell staining
    2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    2.2 Add 1 mL of CFDA-SE working solution, and then incubate at room temperature for 30 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CFSE 関連抗体
    分子量

    557.46

    分子式

    C29H19NO11
    Emission (Em)

    515
    Excitation (Ex)

    485
    CAS 番号
    Appearance

    Solid

    Color

    Off-white to yellow

    SMILES

    CC(OC1=CC=C(C2(C(C=CC(C(ON3C(CCC3=O)=O)=O)=C4)=C4C(O2)=O)C(C=CC(OC(C)=O)=C5)=C5O6)C6=C1)=O.CC(OC7=CC=C(C8(C(C=C(C(ON9C(CCC9=O)=O)=O)C=C%10)=C%10C(O8)=O)C(C=CC(OC(C)=O)=C%11)=C%11O%12)C%12=C7)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (89.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7939 mL 8.9693 mL 17.9385 mL
    5 mM 0.3588 mL 1.7939 mL 3.5877 mL
    10 mM 0.1794 mL 0.8969 mL 1.7939 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 98.23%

    COA (252 KB) HNMR (334 KB) LCMS (262 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献
    細胞実験
    [2]

    Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7939 mL 8.9693 mL 17.9385 mL 44.8463 mL
    5 mM 0.3588 mL 1.7939 mL 3.5877 mL 8.9693 mL
    10 mM 0.1794 mL 0.8969 mL 1.7939 mL 4.4846 mL
    15 mM 0.1196 mL 0.5980 mL 1.1959 mL 2.9898 mL
    20 mM 0.0897 mL 0.4485 mL 0.8969 mL 2.2423 mL
    25 mM 0.0718 mL 0.3588 mL 0.7175 mL 1.7939 mL
    30 mM 0.0598 mL 0.2990 mL 0.5980 mL 1.4949 mL
    40 mM 0.0448 mL 0.2242 mL 0.4485 mL 1.1212 mL
    50 mM 0.0359 mL 0.1794 mL 0.3588 mL 0.8969 mL
    60 mM 0.0299 mL 0.1495 mL 0.2990 mL 0.7474 mL
    80 mM 0.0224 mL 0.1121 mL 0.2242 mL 0.5606 mL
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    CFDA-SE Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CFDA-SE150347-59-4CFSE 5(6)-Carboxyfluorescein diacetate succinimidyl ester 5(6)-CFDA N-succinmidyl esterFluorescent DyeCell Proliferation Fluorescent ProbeInhibitorinhibitorinhibit

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    製品名:
    CFDA-SE
    製品番号:
    HY-D0938
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