Chondroitinase ABC  (Synonyms: ChABC)

Chondroitinase ABC (ChABC) is an enzyme that degrades glycosaminoglycan side-chains of chondroitin sulfate (CS-GAG) from the chondroitin sulfate proteoglycan (CSPG) core protein. Chondroitinase ABC facilitates reinnervation by degrading CS-GAGs around motoneurons. Chondroitinase ABC has the potential for the research of spinal injury^[1][2].

Product Information
The molecular weight of chondroitinase ABC is ~120 kDa (gel filtration and sucrose gradient ultracentrifugation), and SDS-PAGE shows two different subunits with molecular weights of 86 kDa and 32 kDa, respectively. The optimal reaction conditions are pH 8.0 (chondroitin sulfate), pH 6.8 (hyaluronic acid), and 37°C.
Activator: 0.05 M acetate
Inhibitor: 1 mM Zn²⁺

Instructions
Redissolve in 0.01% BSA aqueous solution. Subsequently, dilute with a buffer containing 50 mM Tris HCl (pH 8.0), 60 mM sodium acetate, and 0.02% BSA, and prepare the solution before use.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9024-13-9

Solid

White to off-white

[Chondroitinase ABC]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mahajan R, et al. Chondroitinase ABC Enzyme: A Potential Treatment Option for Spinal Cord Injury. Int J Appl Basic Med Res. 2018 Oct-Dec;8(4):203.

  [2]. Takiguchi M, et al. Chondroitinase ABC Administration Facilitates Serotonergic Innervation of Motoneurons in Rats With Complete Spinal Cord Transection. Front Integr Neurosci. 2022 Jun 30;16:881632.  [Content Brief]