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  3. DBCO-Cy3

DBCO-Cy3  (Synonyms: DBCO-Sulfo-Cy3)

Cat. No.: HY-D1069 Purity: 98.00%
COA Handling Instructions

DBCO-Cy3 (DBCO-Sulfo-Cy3) is the derivative of Cyanine3 fluorophore, a pH insensitive from pH (4-10) orange fluorescent dyewith excitation maximum 555 nm and emission maximum of 580nm. DBCO-Cy3 has fast reaction kinetics and good stability, and is productive to use in many standard fluorescent instrumentations. DBCO-Cy3 is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

For research use only. We do not sell to patients.

DBCO-Cy3 Chemical Structure

DBCO-Cy3 Chemical Structure

CAS No. : 1782950-79-1

Size Price Stock Quantity
5 mg USD 520 In-stock
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50 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

DBCO-Cy3 (DBCO-Sulfo-Cy3) is the derivative of Cyanine3 fluorophore, a pH insensitive from pH (4-10) orange fluorescent dyewith excitation maximum 555 nm and emission maximum of 580nm. DBCO-Cy3 has fast reaction kinetics and good stability, and is productive to use in many standard fluorescent instrumentations. DBCO-Cy3 is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC50 & Target

Orange fluorescent dye

In Vitro

DBCO-Cy3 exhibits less background than TAMRA and most other commonly used fluorescent dyes.
DBCO-Cy3 can be conjugated to antibodies, peptides, and proteins, it can be excited using the 532 nm or 555 nm laser line and visualized with TRITC filter sets.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

983.18

Formula

C50H54N4O11S3

CAS No.
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

570

Excitation (Ex)

550

SMILES

O=S(CCC[N+]1=C(/C=C/C=C2N(CCCCCC(NCCC(N3C4=CC=CC=C4C#CC5=CC=CC=C5C3)=O)=O)C6=C(C=C(S(=O)(O)=O)C=C6)C/2(C)C)C(C)(C)C7=C1C=CC(S(=O)(O)=O)=C7)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (63.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (50.86 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0171 mL 5.0855 mL 10.1711 mL
5 mM 0.2034 mL 1.0171 mL 2.0342 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.00%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.0171 mL 5.0855 mL 10.1711 mL 25.4277 mL
5 mM 0.2034 mL 1.0171 mL 2.0342 mL 5.0855 mL
10 mM 0.1017 mL 0.5086 mL 1.0171 mL 2.5428 mL
15 mM 0.0678 mL 0.3390 mL 0.6781 mL 1.6952 mL
20 mM 0.0509 mL 0.2543 mL 0.5086 mL 1.2714 mL
25 mM 0.0407 mL 0.2034 mL 0.4068 mL 1.0171 mL
30 mM 0.0339 mL 0.1695 mL 0.3390 mL 0.8476 mL
40 mM 0.0254 mL 0.1271 mL 0.2543 mL 0.6357 mL
50 mM 0.0203 mL 0.1017 mL 0.2034 mL 0.5086 mL
DMSO 60 mM 0.0170 mL 0.0848 mL 0.1695 mL 0.4238 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DBCO-Cy3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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