ddGTP tetrasodium  (Synonyms: 2′,3′-Dideoxyguanosine 5′-triphosphate tetrasodium)

ddGTP (2′,3′-Dideoxyguanosine 5′-triphosphate) tetrasodium is one of 2',3'-dideoxyribonucleoside 5'-triphosphates (ddNTPs) that acts as chain-elongating inhibitor of DNA polymerase for DNA sequencing. ddGTP tetrasodium acts as an inhibitor or a substrate for DNA polymerase α^[1][2][3].

ddGTP tetrasodium inhibits the formation of both negative and positive DNA strands^[1].
ddGTP tetrasodium inhibits DNA polymerase α activity in the presence of Mn²⁺ at low concentrations (<1 μM)^[2].
ddGTP tetrasodium competitively inhibits the incorporation of [3H]dGTP into the DNA^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

579.11

C₁₀H₁₂N₅Na₄O₁₂P₃

O=[P](O[P](O[P](O[Na])(O[Na])=O)(O[Na])=O)(O[Na])OC[C@@H]1CC[C@H](N2C(NC(N)=N3)=C(N=C2)C3=O)O1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Debyser Z, et al. Kinetics of inhibition of endogenous human immunodeficiency virus type 1 reverse transcription by 2',3'-dideoxynucleoside 5'-triphosphate, tetrahydroimidazo-[4,5,1-jk][1,4]-benzodiazepin-2(1H)-thion e, and 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine derivatives. J Biol Chem. 1992 Jun 15;267(17):11769-76.  [Content Brief]

  [2]. Ono K, Nakane H. Utilization of 2',3'-dideoxyguanosine 5'-triphosphate as an inhibitor and substrate for DNA polymerase alpha. Biomed Pharmacother. 1990;44(2):115-21.  [Content Brief]

  [3]. Z G Chidgeavadze, et al. 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8.  [Content Brief]