2. Fluorescent Dye
  3. Cell Behavior analysis Probes

Cell Behavior analysis Probes

Cells exhibit dynamic behaviors during proliferation, migration, differentiation and apoptosis, directly influencing tissue development, immune responses and disease progression. Cell function analysis dyes enable real-time monitoring of the dynamics of key structures or molecules, providing a convenient and intuitive tool for cellular proliferation investigation and active species detection.The fluorescent stock solutions are easy to use with minimized reagent loss, while ensuring experimental reproducibility.

Cell Behavior analysis Probes (30):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-DY1002
    H2DCFDA (solution)
    H2DCFDA (DCFH-DA) (solution) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) .
    Solvent and concentration: DMSO: 10 mM.
    The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    H2DCFDA (solution)
  • HY-DY1014
    ATP-Red 1 (solution) 1847485-97-5
    ATP-Red 1 (solution) is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).
    Solvent and concentration: DMSO: 1 mM
    ATP-Red 1 (solution)
  • HY-DY1011
    PKH 26 (solution) 154214-55-8
    PKH 26 (solution) is a red fluorescent dye, PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm) , which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies.
    Solvent and concentration: DMSO: 5 mM
    PKH 26 (solution)
  • HY-DY1036
    Calcein-AM (solution) 148504-34-1
    Calcein AM (solution) , has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein) , which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm.
    Solvent and concentration: DMSO: 2 mM
    Calcein-AM (solution)
  • HY-DY1046
    Green CMFDA (solution) 136832-63-8
    Green CMFDA (solution) is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Solvent and concentration: DMSO: 10 mM
    Green CMFDA (solution)
  • HY-DY1102
    BODIPY FL-C16 (solution) 158757-82-5
    BODIPY FL-C16 (solution) is a BODIPY-labeled analog of Palmitic acid (HY-N0830), which serves as a fluorescent lipid tracer. BODIPY FL-C16 (solution) also acts as a ligand for liver fatty acid-binding protein (L-FABP) and intestinal fatty acid-binding protein (I-FABP) , with Kd values of 270 nM and 330 nM, respectively. BODIPY FL-C16 (solution) is rapidly taken up by cells, and after metabolic conversion to phospholipids, it is incorporated into the membrane structures of intracellular organelles and extracellular vesicles.
    Solvent and concentration: DMSO: 5 mM
    BODIPY FL-C16 (solution)
  • HY-DY1019
    2-NBDG (solution) 186689-07-6
    2-NBDG (solution) is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
    Solvent and Concentration: Sterile PBS: 5 mM
    2-NBDG (solution)
  • HY-DY1015
    Dihydroethidium (solution) 104821-25-2
    Dihydroethidium (solution) , also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation.
    Solvent and concentration: DMSO: 5 mM
    Dihydroethidium (solution)
  • HY-DY1048
    DAF-FM DA (solution) 254109-22-3
    DAF-FM DA (Diaminofluorescein-FM diacetate) (solution) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm) .
    Solvent and concentration: DMSO: 2 mM
    DAF-FM DA (solution)
  • HY-DY1059
    FM1-43 (solution) 149838-22-2
    FM1-43 (solution) is a very lipophilic, water-soluble styrene dyes, can specifically bind to cell membranes and inner membrane organelles to produce fluorescence. FM1-43 is widely used in endocytic and exospic membrane structure markers.
    Solvent and concentration: DMSO: 10 mM
    FM1-43 (solution)
  • HY-DY1009
    CFDA-SE (solution) 150347-59-4
    CFDA-SE (solution) is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.
    Solvent and concentration: DMSO: 5 mM
    CFDA-SE (solution)
  • HY-DY1035
    Hydroxyphenyl Fluorescein (solution) 359010-69-8
    Hydroxyphenyl Fluorescein (HPF) (solution) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm.
    Solvent and concentration: DMSO: 10 mM
    Hydroxyphenyl Fluorescein (solution)
  • HY-DY1016
    FITC-Dextran (MW 10000) (solution) 60842-46-8
    FITC-Dextran (MW 10000) (solution) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.
    Solvent and Concentration: Sterile water: 2 mM
    FITC-Dextran (MW 10000) (solution)
  • HY-DY1044
    HADA hydrochloride (solution) 2253733-10-5
    HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation.
    Solvent and concentration: DMSO: 50 mM
    HADA hydrochloride (solution)
  • HY-DY1037
    Dihydrorhodamine 123 (solution) 109244-58-8
    Dihydrorhodamine 123 (DHR 123) (solution) is a non-fluorescent reactive oxygen species (ROS) indicator. Dihydrorhodamine 123 is oxidized to fluorescent Rhodamine 123 (HY-D0816) within cells in the presence of reactive oxygen species and it localizes in mitochondria (Ex/Em = 515/536 nm).
    Solvent and concentration: DMSO: 10 mM
    Dihydrorhodamine 123 (solution)
  • HY-DY1034
    4-MUNANA (solution) 76204-02-9
    4-MUNANA (solution) is a substrate of influenza virus neuraminidase (NA) with high selectivity and irreversible reaction. In the enzymatic reaction, 4-MUNANA is hydrolyzed by NA to generate fluorescent 4-methylumbelliferone (4-MU). By detecting the fluorescence intensity of 4-MU, quantitative analysis of NA activity can be achieved. 4-MUNANA can be used in influenza-related research, such as screening NA inhibitors, developing new anti-influenza drugs, and studying the infection mechanism of influenza viruses.
    Solvent and Concentration: Sterile water: 10 mM
    4-MUNANA (solution)
  • HY-DY1100
    WST-1 (solution) 150849-52-8
    WST-1 (solution) is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm.
    Solvent and concentration: ddH2O: 10 mg/mL
    WST-1 (solution)
  • HY-DY1033
    C12FDG (solution) 138777-25-0
    C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) (solution) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di (β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm) .
    Solvent and concentration: DMSO: 10 mM
    C12FDG (solution)
  • HY-DY1041
    CellTracker Blue CMAC (solution) 147963-22-2
    CellTracker Blue CMAC (solution) is a non-fluorescent cell membrane permeable dye. The chloromethyl groups of CellTracker Blue CMAC are enzymatically cleaved by intracellular glutathione (GSH) to generate a fluorescent product (blue fluorescence, Ex/Em: 360/460 nm). CellTracker Blue CMAC is suitable for long-term cell tracking (up to 72 hours) and cell proliferation studies, and can also quantify GSH levels.
    Solvent and concentration: DMSO: 10 mM
    CellTracker Blue CMAC (solution)
  • HY-DY1018
    FITC-Dextran (MW 4000) (solution) 60842-46-8
    FITC-Dextran (MW 4000) (solution) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 4000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 4000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.
    Solvent and Concentration: Sterile water: 2 mM
    FITC-Dextran (MW 4000) (solution)