PKH 26 (solution) 

PKH 26 (solution) is a red fluorescent dye, PKH 26 can stably bind to the lipid region of cell membrane and emit red fluorescence (Ex/Em=551/567 nm) , which is mainly used for in vitro cell labeling, in vitro cell proliferation studies and in vivo and in vitro cell tracing studies^[1].
Solvent and concentration: DMSO: 5 mM

Guidelines (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.)
General Protocol
1.1 Preparation of PKH 26 Working Solution
Dilute with serum-free cell culture medium or PBS. Adjust the stock solution as needed. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: Adjust the concentration of PKH 26 working solution as needed.
2. Cell Staining
2.1 Suspension Cells (6-well plate)
a. Centrifuge at 4°C, 1000 g for 3-5 minutes, discard the supernatant, wash twice with PBS for 5 minutes each time. Cell density is 1×10?/mL.
b. Add 1 mL of working solution and incubate at room temperature for 10-45 minutes.
c. Centrifuge at 400 g for 3-4 minutes at 4°C and discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend the cells in serum-free cell culture medium or PBS. Observe under a fluorescence microscope or flow cytometry.
2.2 Adherent Cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslips from the culture medium and aspirate any excess medium.
c. Add 100 μL of working solution, gently shake to completely cover the cells, and incubate at room temperature for 5-30 minutes.
d. Wash twice with culture medium, 5 minutes each time. Observe under a fluorescence microscope or flow cytometry.

Storage
It is recommended to store the stock solution at -80°C, protected from light, and avoid repeated freeze-thaw cycles.
Protect from light

Precautions
1. Please adjust the concentration of PKH 26 working solution according to actual conditions.
2. This product is for research and development use only and must not be used for pharmaceutical, household, or other purposes.
3. For your safety and health, please wear a white coat and disposable gloves when handling.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

961.34

C₅₉H₉₇IN₂

154214-55-8

Liquid

Pink to red

CC1(C)C(/C=C/C=C2N(CCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)=[N+](CCCCCCCCCCCCCCCCCCCCCC)C4=C1C=CC=C4.[I-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Fischer K, et al. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity. Methods. 2003;31(2):135-142.  [Content Brief]