2. GPCR/G Protein
  3. Endothelin Receptor
  4. Endothelin 1 (swine, human) TFA

Endothelin 1 (swine, human) TFA  (Synonyms: ET-1 (swine, human) TFA)

製品番号: HY-P0202A 純度: 99.77%
COA 取扱説明書 Technical Support

Endothelin 1 (swine, human) (ET-1) TFA is a synthetic peptide with the sequence of human and swine Endothelin 1, which is a potent endogenous vasoconstrictor. Endothelin 1 TFA acts through two types of receptors ETA and ETB.

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CAS 番号 : 394693-38-0

容量 価格（税別） 在庫状況 数量
500 μg $144 在庫あり
5 mg $850 在庫あり
10 mg $1360 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

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カスタマーレビュー

Based on 8 publication(s) in Google Scholar

Other Forms of Endothelin 1 (swine, human) TFA:

Endothelin 1 (swine, human) TFA 関連抗体

Top Publications Citing Use of Products

    Endothelin 1 (swine, human) TFA purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Sep 21;14(1):5891.  [Abstract]

    Endothelin 1 (ET-1, 100 n mol/L, 6–36 h) markedly decreased Sp1 and Sp3 expression in HUVECs.

    Endothelin 1 (swine, human) TFA purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Sep 21;14(1):5891.  [Abstract]

    Chromatin immunoprecipitation (ChIP) assay showing the binding of Sp1 or Sp3 to the AMPKα1 and AMPKα2 promoter in HUVECs with different treatments. n = 3. The results showed that Endothelin 1 (ET-1,100 nmol/L) reduced the Sp1/Sp3 association with AMPKα1 and AMPKα2 promoters.

    Endothelin 1 (swine, human) TFA purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Jan 5:962:176236.  [Abstract]

    Endothelin-1 (ET-1, 100 nmol/L; 24 h) significantly decreased the proportion of apoptotic porcine GCs under both normoxic (P < 0.05) (Fig. 6c) and hypoxic conditions (P < 0.01).

    Endothelin 1 (swine, human) TFA purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2024 Jan 5:962:176236.  [Abstract]

    Nuclear‒cytoplasmic fractionation was performed to analyze FOXO1 expression in the cytoplasm and nucleus after porcine GCs were treated with Endothelin-1 (ET-1, 100 nmol/L) for 24 h. n = 3 biological replicates per group. The results showed that ET-1 significantly reduced nuclear FOXO1 expression under both normoxic (P < 0.01) and hypoxic (P < 0.001) conditions.

    Endothelin 1 (swine, human) TFA purchased from MedChemExpress. Usage Cited in: Sci Adv. 2022 Jul 29;8(30):eabn4408.  [Abstract]

    Pharmacogenetic suppression of the VLO significantly decreased the number of scratching bouts induced by intradermal injection of Endothelin-1 (cheek; nape) (20.00 μM; unilateral intradermal injection) following CNO administration (4 mg/kg, ip). n = 7 to 8 rats in each group.

    Endothelin Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ETA ETB

    • 生物活性

    • 純度とドキュメンテーション

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    製品説明

    Endothelin 1 (swine, human) (ET-1) TFA is a synthetic peptide with the sequence of human and swine Endothelin 1, which is a potent endogenous vasoconstrictor. Endothelin 1 TFA acts through two types of receptors ETA and ETB[1].

    体外実験

    Endothelin-1 TFA is a potent endogenous vasoconstrictor, mainly secreted by endothelial cells. Endothelin-1 TFA acts through two types of receptors: ETA and ETB. Apart from a vasoconstrictive action, Endothelin-1 TFA causes fibrosis of the vascular cells and stimulates production of reactive oxygen species. Endothelin-1 TFA induces proinflammatory mechanisms, increasing superoxide anion production and cytokine secretion[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Endothelin 1 (swine, human) TFA 関連抗体
    体内実験

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    1. Induction of Post-stroke Depression (PSD)[2][3][4]
    Background
    Unilateral Endothelin-1 TFA injection in the mouse mPFC induces a discrete, reproducible ischemic lesion via acute vasoconstriction and reduced blood flow in the target area, producing a persistent post-stroke depression (PSD) phenotype (anxiety/depression) without sensorimotor deficits[3].
    Specific Modeling Methods
    Mice: C57BL/6J mice • 8-12 weeks • 22-35 g
    Administration: Endothelin 1 (2 mg/mL in 1 μL saline) TFA • microinjected into the unilateral (right) mPFC of mice using stereotaxic coordinates derived from the brain atlas by Paxinos and Watson (Site 1: anterior-posterior (AP): +2.0 mm, medial-lateral (ML): -0.5 mm, dorsoventral (DV): -2.4 mm; Site 2: AP: +1.5 mm, ML: -0.5 mm, DV: -2.6 mm)[2]
    Mice: C57BL/6 mice • 10-11 weeks • 25-28 g
    Administration: Endothelin 1 (2 μg/μL in 1 μL sterile water) TFA • microinjected at two sites within the left mPFC at the following coordinates (in mm) relative to Bregma48. Injection 1, anterior-posterior (AP), 2.0; medial-lateral (ML), +0.5; dorsoventral (DV), -2.4; Injection 2, AP 1.5; ML +0.5; DV -2.6[3].
    Rats: Adult male Sprague-Dawley rats
    Administration: Endothelin 1 (400 pmol in 1 μL) TFA • microinjected at the following coordinates: anterior/posterior +3.0, medial/lateral -1.3, and dorsal/ventral -3.5 (all coordinates relative to bregma)[4]
    Note
    (1) A microsyringe (10 μL) was used to load 1 μL of pre-dissolved Endothelin 1 TFA, which was injected at a rate of 100 nL/min over 10 minutes at the designated coordinates[2].
    (2) After the injection, the syringe needle was left in place for 10 minutes to prevent backflow, then withdrawn slowly, and the scalp was sutured[2].
    (3) An infusion pump was used to load 1 μL of pre-dissolved Endothelin 1 TFA, which was set at a rate of 0.20 μL/min[3].
    (4) At the end of the infusion, the needle was left in place for 3 min before gradual withdrawal and closure of the incision that was treated with 0.1 ml of 2% transdermal bupivacaine as a topical anesthetic[3].
    (5) Antibiotics were applied to the sutured area to prevent postoperative infection, and the mice were placed on a thermostatic heating pad to maintain body ature until full recovery[2][3].
    Modeling Indicators
    Histology analysis: Focal ischemic lesions at injection site (48 h post-surgery)
    Behavioral changes: Reduced center exploration (open field test, OFT); Decreased time/distance in the open arms (elevated plus-maze, EPM); Increased immobility (forced swimming test, FST; tail suspension test, TST); Reduced sucrose preference (sucrose preference test, SPT); Prolonged latency (novelty-suppressed feeding, NSF); No significant impairment in locomotion/motor coordination (OFT total distance, Rotarod, Cylinder test)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    2605.92

    分子式

    C111H160F3N25O34S5
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp (Disulfide bridge: Cys1-Cys15, Cys3-Cys11)
    Sequence Shortening

    CSCSSLMDKECVYFCHLDIIW (Disulfide bridge: Cys1-Cys15, Cys3-Cys11)
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    polypeptide, sealed storage, away from moisture and light, under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light, under nitrogen)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (19.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 20 mg/mL (7.67 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.3837 mL 1.9187 mL 3.8374 mL
    5 mM 0.0767 mL 0.3837 mL 0.7675 mL
    10 mM 0.0384 mL 0.1919 mL 0.3837 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.77%

    COA (245 KB) LCMS (94 KB) Elemental Analysis Report (105 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.3837 mL 1.9187 mL 3.8374 mL 9.5935 mL
    5 mM 0.0767 mL 0.3837 mL 0.7675 mL 1.9187 mL
    DMSO 10 mM 0.0384 mL 0.1919 mL 0.3837 mL 0.9594 mL
    15 mM 0.0256 mL 0.1279 mL 0.2558 mL 0.6396 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Endothelin 1 (swine, human) TFA Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Endothelin 1 (swine, human)394693-38-0ET-1 (swine, human)Endothelin ReceptorVasoconstrictorEndothelinETAETBPeptideInhibitorinhibitorinhibit

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    製品番号:
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