2. Others Apoptosis Cell Cycle/DNA Damage
  3. Biochemical Assay Reagents Apoptosis DNA Alkylator/Crosslinker
  4. Ethyl methanesulfonate

メタンスルホン酸エチル  (Synonyms: Ethyl methanesulfonate)

製品番号: HY-W015854 純度: 98.33%
COA 取扱説明書 Technical Support

Ethyl methanesulfonate is an orally active biochemical agent. Ethyl methanesulfonate induces Apoptosis. Ethyl methanesulfonate acts on DNA, alkylating it and causing changes in DNA structure, which in turn triggers a series of biological effects such as mutation and cell death. Ethyl methanesulfonate induces kidney and nervous system tumors. Ethyl methanesulfonate is widely used in the field of genetic toxicology research and is often used to induce gene mutations in organisms to study gene function, the mechanism of genetic diseases, and the effects of environmental mutagenic factors, etc.

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Ethyl methanesulfonate

メタンスルホン酸エチル 構造式

CAS 番号 : 62-50-0

容量 価格（税別） 在庫状況 数量
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Liquid
1 g $25 在庫あり
5 g $40 在庫あり
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Other Forms of Ethyl methanesulfonate:

メタンスルホン酸エチル 関連抗体

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製品説明

Ethyl methanesulfonate is an orally active biochemical agent. Ethyl methanesulfonate induces Apoptosis. Ethyl methanesulfonate acts on DNA, alkylating it and causing changes in DNA structure, which in turn triggers a series of biological effects such as mutation and cell death. Ethyl methanesulfonate induces kidney and nervous system tumors. Ethyl methanesulfonate is widely used in the field of genetic toxicology research and is often used to induce gene mutations in organisms to study gene function, the mechanism of genetic diseases, and the effects of environmental mutagenic factors, etc[1][2][3][4][5][6][7][8][9][10].

Cellular Effect
Cell Line Type Value Description References
CHO-AA8 IC50
6800 nM
Compound: EMS
Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)
Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)
[PMID: 2909741]
体外実験

Ethyl methanesulfonate (0-3 mM) causes a decrease in cell viability, an increase in apoptosis, and inhibits cell growth and cloning efficiency in AHH-1 human lymphoblastoid cell line[2].
Ethyl methanesulfonate (30 mM; 0-5 h) causes cell death, lipid peroxidation, and a decrease in mitochondrial membrane potential, intracellular ATP and K+ concentrations in human hepatocyte[4].
Ethyl methanesulfonate (1-30 mM; 2 h) induces DNA damage in the single-cell gel electrophoresis (SCGE) experiment of tobacco cell line TX1, and the degree of damage is positively correlated with the concentration and treatment time[5].
Ethyl methanesulfonate (10-250 μM; 1 h) can dose-dependently form N-ethyl-valinehemoglobin adducts in in vitro experiments with human hemoglobin (Hb)[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

メタンスルホン酸エチル 関連抗体

Cell Viability Assay[2]

Cell Line: AHH-1 human lymphoblastoid cell line
Concentration: 0 mM, 1 mM, 2 mM, 3 mM
Incubation Time: 0 day, 2 days, 3 days, 7 days
Result: Decreased the number of viable cells.
Did not significantly affect the relative cell growth, but the length of the interval between exposure and cell count had a significant effect on cell count.
体内実験

Ethyl methanesulfonate (50-400 mg/kg; i.p. and p.o.; single dose) significantly induces the production of micronucleated polychromatic erythrocytes (MNPCEs) in the micronucleus test of MS/Ae and CD-1 mice, showing an obvious dose-response relationship, and the induction rates of the two administration routes are similar[7].
Ethyl methanesulfonate (25-100 mg/kg/day; oral administration; once a day; 28 days) significantly increases the mutant frequency of the Pig-a gene in male F344 rats in the Pig-a assay after 2 and 4 weeks of dosing[8].
Ethyl methanesulfonate (multiple doses of 27.5 mg; intraperitoneal injection; multiple times; no clear administration cycle) induces a high incidence of primary kidney tumors in female Wistar albino rats[9].
Ethyl methanesulfonate (250 mg/kg; i.p.; single dose) causes unscheduled DNA synthesis in meiotic and postmeiotic germ cell stages of male (C3Hf×101)F1 mice[10].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Wistar albino rats (100 g at the start of the experiment), tumor induction model[9]
Dosage: Three separate intraperitoneal doses of 27.5 mg (dissolved in 1 ml 0.9% sodium chloride)
Administration: Intraperitoneal injection, the second dose was given 2 days after the first, and the third 7 days later
Result: Produced kidney tumors in twelve rats.
Showed two of the rats had tumors in both kidneys.
Showed that histologically, all the tumors were similar and indistinguishable from those induced by dimethylnitrosamine, N-methyl-N-nitrosourea, and the related carcinogen eyeasin.
分子量

124.15

分子式

C3H8O3S
CAS 番号
Appearance

Liquid (Density: 1.206 g/cm3)

Color

Colorless to light yellow

SMILES

CS(=O)(OCC)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Pure form -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 100 mg/mL (805.48 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.0548 mL 40.2739 mL 80.5477 mL
5 mM 1.6110 mL 8.0548 mL 16.1095 mL
10 mM 0.8055 mL 4.0274 mL 8.0548 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.96%

COA (252 KB) HNMR (112 KB) GC (187 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 8.0548 mL 40.2739 mL 80.5477 mL 201.3693 mL
5 mM 1.6110 mL 8.0548 mL 16.1095 mL 40.2739 mL
10 mM 0.8055 mL 4.0274 mL 8.0548 mL 20.1369 mL
15 mM 0.5370 mL 2.6849 mL 5.3698 mL 13.4246 mL
20 mM 0.4027 mL 2.0137 mL 4.0274 mL 10.0685 mL
25 mM 0.3222 mL 1.6110 mL 3.2219 mL 8.0548 mL
30 mM 0.2685 mL 1.3425 mL 2.6849 mL 6.7123 mL
40 mM 0.2014 mL 1.0068 mL 2.0137 mL 5.0342 mL
50 mM 0.1611 mL 0.8055 mL 1.6110 mL 4.0274 mL
60 mM 0.1342 mL 0.6712 mL 1.3425 mL 3.3562 mL
80 mM 0.1007 mL 0.5034 mL 1.0068 mL 2.5171 mL
100 mM 0.0805 mL 0.4027 mL 0.8055 mL 2.0137 mL
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Ethyl methanesulfonate Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethyl62-50-0Biochemical Assay ReagentsApoptosisDNA Alkylator/Crosslinkerbiochemical reagentbiological materialorganic compoundlife scienceInhibitorinhibitorinhibit

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