inS3-54A18
Based on 1 Customer Validation
inS3-54A18 is a potent STAT3 inhibitor, with anti-cancer properties.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 328998-53-4
- Formula: C23H16ClNO2
- Molecular Weight:373.83
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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JAK/STAT Compound Library
HY-L008
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Kinase Inhibitor Library
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Stem Cell Signaling Compound Library
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Anti-Aging Compound Library
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Anti-Liver Cancer Compound Library
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Anti-Prostate Cancer Compound Library
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Posttranslational Modification Library
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Lactylation Compound Library
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Biological Activity
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STAT3 |
inS3-54A18 (5 μM) reduces wound healing to 64% and 76% for A549 and MDA-MB-231 cells, respectively. inS3-54A18 at 10 μM further reduces the healing to 47% and 39%, respectively. inS3-54A18 inhibits the expression of STAT3 downstream target genes and the binding of STAT3 to its endogenous target sequences. inS3-54A18 does not affect the constitutive or IL-6-induced STAT3 activation (Tyr705 phosphorylation) but represses STAT3 target gene (survivin) expression[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 328998-53-4
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Appearance Solid
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Molecular Weight 373.83
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Formula C23H16ClNO2
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Color Yellow to orange
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SMILES
O=C1N(C2=CC=C(O)C=C2)C(C3=CC=CC=C3)=C/C1=C/C4=CC=C(Cl)C=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (267.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Briefly, 5×106 A549 cells are injected subcutaneously in the flanks of 5-6-week-old male NOD/SCID mice. When the tumor volume reach about 50.0 mm3, the mice are randomized using online program into two different groups (6/group) with one group treated by formulation vehicle control and the other by A18 at 200 mg/kg with oral dosing 2-3 times a week for 4 weeks. One mouse in each group dies during the study and is eliminated. Tumor volume and body weight of the remaining five mice in each group are measured twice a week without blinding. On the thirty-fifth day after implant, mice are euthanized and the tumor tissues are harvested and weighed. Necropsy is also performed to determine changes in the heart, lungs, kidneys, liver and spleen.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6750 mL | 13.3751 mL | 26.7501 mL | 66.8753 mL |
| 5 mM | 0.5350 mL | 2.6750 mL | 5.3500 mL | 13.3751 mL | |
| 10 mM | 0.2675 mL | 1.3375 mL | 2.6750 mL | 6.6875 mL | |
| 15 mM | 0.1783 mL | 0.8917 mL | 1.7833 mL | 4.4584 mL | |
| 20 mM | 0.1338 mL | 0.6688 mL | 1.3375 mL | 3.3438 mL | |
| 25 mM | 0.1070 mL | 0.5350 mL | 1.0700 mL | 2.6750 mL | |
| 30 mM | 0.0892 mL | 0.4458 mL | 0.8917 mL | 2.2292 mL | |
| 40 mM | 0.0669 mL | 0.3344 mL | 0.6688 mL | 1.6719 mL | |
| 50 mM | 0.0535 mL | 0.2675 mL | 0.5350 mL | 1.3375 mL | |
| 60 mM | 0.0446 mL | 0.2229 mL | 0.4458 mL | 1.1146 mL | |
| 80 mM | 0.0334 mL | 0.1672 mL | 0.3344 mL | 0.8359 mL | |
| 100 mM | 0.0268 mL | 0.1338 mL | 0.2675 mL | 0.6688 mL |
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