2. MCE-Kits
  3. Molecular Biology
  4. Nucleic Acid Gel Electrophoresis
  5. Agarose
  6. Agarose

Agarose 

Art. -Nr.: HY-K1031
Manual COA SDS Technical Support
Expression Purification

MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.

Größe Preis Verfügbarkeit Menge
Kostenlose Probe (10 g)   Jetzt bewerben  
100 g Auf Lager
or Massenanfrage

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  • Beschreibung

  • Speicherung

  • Protokoll

  • Komponenten

  • Dokumentation

Description
& Advantages

Agarose is widely used for nucleic acid isolation and identification. This product can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.


Features
Gel Strength (1% gel) > 1,200 g/cm2
Electroosmosis (EEO) < 0.15
Sulfide ≤ 0.15%
Gel Point (1.5% gel) 35-37°C
Melting Point (1.5% gel) 87-89°C
Water concentration ≤ 10%
Nuclease None
Speicherung

Store at room temperature for 3 years.

Protokoll

1. Choose appropriate agarose concentration according to the size of nucleic acid fragment and the type of electrophoresis buffer.

2. Prepare the gel

a. Add appropriate agarose into the cleaned conical flask and dissolve with appropriate electrophoresis buffer (TAE or TBE). Seal the flask and heat in the microwave until the agarose is completely dissolved.

b. Add Nucleic Acid dye (HY-K1004 MCE SYBR Green I Nucleic Acid Gel Stain).

c. Mix gently and pour the agarose solution into the rubber board and insert the comb in place. The thickness of the gel is generally 3-5 mm.

d. Cool at room temperature for 30-60 minutes for subsequent electrophoresis.
Komponenten
Components HY-K1031-100 g
Agarose 100 g
Dokumentation

Manual (164 KB) SDS (365 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Agarose gel electrophoresisAgarose gel

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