1. MCE Kits
  2. Cell Biology
  3. Cell Isolation
  4. Mouse CD8+ T Cells Negative Selection Kit

Mouse CD8+ T Cells Negative Selection Kit 

Cat. No.: HY-K0309
Manual COA SDS Technical Support

MCE Mouse CD8+ T Cells Negative Selection Kit is designed for the isolation of CD8+ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

사이즈 가격 재고 수량
1 mL 해외재고보유
2 mL 해외재고보유

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

    MedChemExpress Validation
    CD8+ cells were sorted from mouse splenocytes using the Mouse CD8+ T Cells Negative Selection Kit (HY-K0309). CD8+ T cells were isolated from C57BL/6 mouse splenocytes and stained with FITC-conjugated anti-mouse CD8 antibody (clone 53-6.7) before and after sorting for flow cytometric analysis. The results showed that the purity of CD8+ T cells increased from 12.7% (pre-sorting) to 94.8% (post-sorting).

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    Description
    & Advantages

    MCE Mouse CD8+ T Cells Negative Selection Kit is designed for the isolation of CD8+ T cells from single cell suspensions of mouse spleen cells and lymph nodes. Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD8+ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD8+ T cells.

    The kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

    Features of MCE Mouse CD8+ T Cells Negative Selection Kit

    •   Efficiency: Enables sorting of target cells in as little as 15 minutes;

    •   Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

    •   High Purity: Can yield sorted cells with a purity exceeding 97%;

    •   High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.

    보관

    4°C,2 years

    Do not freeze the magnetic beads

    구성요소
    Components HY-K0309-1 mL (For 5 × 108 cells) HY-K0309-2 mL (For 109 cells)
    Biotin-Antibody Mix 100 μL 200 μL
    Streptavidin Magnetic Beads 1 mL 1 mL × 2
    각종 서류

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    상품명:
    Mouse CD8+ T Cells Negative Selection Kit
    Cat. No.:
    HY-K0309
    수량:
    MCE Japan Authorized Agent: