2. MCE-Kits
  3. Molecular Biology
  4. Vector Construction
  5. Seamless Cloning
  6. Seamless DNA Assembly Plus Kit

Seamless DNA Assembly Plus Kit 

Art. -Nr.: HY-K1041
Manual COA SDS Technical Support
Expression Purification

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.

Größe Preis Verfügbarkeit
50 rxns Anfrage über Preis & Lieferzeit

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

  • Beschreibung

  • Speicherung

  • Komponenten

  • Dokumentation

Description
& Advantages

Based on gene recombination, seamless cloning is a simple, efficient, and fast DNA cloning technology. A 15-20 bp sequence homologous to the end of the linearized vector is added to the 5′ end of the insert fragment primers. In the presence of enzymes, recombination can be easily achieved.

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.
Speicherung

Store at -20°C for 2 years. Avoid repeated freeze-thaw cycles.

Komponenten
Components HY-K1041-50 rxns
Seamless Assembly Mix Plus 250 μL
pUC19 Control Plasmid, Linearized (Ampr, 40 ng/μL) 5 μL
500 bp Control Fragment (20 ng/μL) 5 μL
Dokumentation

Manual (729 KB) SDS (311 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Seamless Cloning and Assembly KitDNA cloningDNA recombinationDNA assembly

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