Janelia Fluor® 646, Tetrazine  (Synonyms: JF646, Tetrazine)

Janelia Fluor 646, Tetrazine (JF646, Tetrazine) a red fluorescent dye that contains a tetrazine group. JF646, Tetrazine can be used in cellular imaging^[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

Excitation maximum=646 nm; emission maximum=664 nm^[1].
JF646 can serve as ligand for self-labeling tag (such as HaloTag) ^[1].
JF646, Tetrazine is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient^[2]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

665.82

C₃₈H₃₅N₇O₃Si

2042192-00-5

Solid

Pink to red

646

605

O=C(C1=C(C(C2=C([Si](C)(C)C3=C/4)C=C(N5CCC5)C=C2)=C3C=CC4=[N+]6CCC/6)C=C(C(NCC7=CC=C(C8=NN=CN=N8)C=C7)=O)C=C1)[O-]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256  [Content Brief]

  [2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28.  [Content Brief]