2. Academic Validation
  3. Polygalasaponin F against rotenone-induced apoptosis in PC12 cells via mitochondria protection pathway

Polygalasaponin F against rotenone-induced apoptosis in PC12 cells via mitochondria protection pathway

  • J Asian Nat Prod Res. 2014 Jan;16(1):59-69. doi: 10.1080/10286020.2013.864283.
Miao-Miao Wu 1,  Yu-He Yuan,  Jiao Chen,  Chuang-Jun Li,  Dong-Ming Zhang,  Nai-Hong Chen
Affiliations

Affiliation

  • 1 a State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Department of Pharmacology , Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , 100050 , China.
PMID: 24382325 DOI: 10.1080/10286020.2013.864283
Abstract

To investigate the protective effect and the underlying mechanism of polygalasaponin F (PS-F) against rotenone-induced PC12 cells, the cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. The cell Apoptosis rate was analyzed using flow cytometry. The Reactive Oxygen Species was examined using 2',7'-dichlorofluorescin diacetate, and the adenosine triphosphate depletion was examined using a luciferase-coupled quantification assay. JC-1 staining was used to detect the mitochondrial membrane potential. Western blotting analysis was used to determine cytochrome c, p53, Bax, Bcl-2, and Caspase-3. Treatment of PC12 cells with rotenone (1-10 μmol/l) significantly reduced the cell viability in a concentration-dependent manner. Treatment with PS-F (0.1, 1, and 10 μmol/l) increased the viability of rotenone-induced PC12 cells, decreased rotenone-induced Apoptosis, restored rotenone-induced mitochondrial dysfunction, and suppressed rotenone-induced protein expression. PS-F showed a dose-dependent manner in all the treatments. PS-F protects PC12 cells against rotenone-induced Apoptosis via ameliorating the mitochondrial dysfunction. Thus, PS-F may be a potential bioactive compound for the treatment of Parkinson's disease.

Figures
Products