1. Academic Validation
  2. Metabolic response of Lactobacillus acidophilus exposed to amoxicillin

Metabolic response of Lactobacillus acidophilus exposed to amoxicillin

  • J Antibiot (Tokyo). 2022 May;75(5):268-281. doi: 10.1038/s41429-022-00518-6.
Yue Guo 1 Xi Liu 1 Huimin Huang 1 Yating Lu 1 Xue Ling 1 Yiyi Mo 1 Chunli Yin 1 Hongjia Zhu 1 Hua Zheng 1 Yonghong Liang 1 Hongwei Guo 1 Rigang Lu 2 Zhiheng Su 3 Hui Song 4
Affiliations

Affiliations

  • 1 Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
  • 2 Guangxi Institute for Food and Drug Control, Nanning, 530021, China. [email protected].
  • 3 Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China. [email protected].
  • 4 Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China. [email protected].
Abstract

Drug-induced diarrhea is a common adverse drug reaction, especially the one caused by the widespread use of Antibiotics. The reduction of probiotics is one reason for intestinal disorders induced by an oral Antibiotic. However, the intrinsic mechanism of drug-induced diarrhea is still unknown. In this study, we used metabolomics methods to explore the effects of the classic oral Antibiotic, amoxicillin, on the growth and metabolism of Lactobacillus acidophilus, while scanning electron microscopy (SEM) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were employed to evaluate changes in cell activity and morphology. The results showed that cell viability gradually decreased, while the degree of cell wall rupture increased, with increasing amoxicillin concentrations. A non-targeted metabolomics analysis identified 13 potential biomarkers associated with 9 metabolic pathways. The data showed that arginine and proline metabolism, nicotinate and nicotinamide metabolism, pyrimidine metabolism, glycine, serine and threonine metabolism, beta-alanine metabolism, glycerolipid metabolism, tryptophan metabolism, steroid hormone biosynthesis, and histidine metabolism may be involved in the different effects exerted by amoxicillin on L. acidophilus. This study provides potential targets for screening probiotics regulators and lays a theoretical foundation for the elucidation of their mechanisms.

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