1. Academic Validation
  2. Antidiarrheal effect of ethanol extract from Lophatheri Herba and its effect on isolated jejunal smooth muscle in rabbits

Antidiarrheal effect of ethanol extract from Lophatheri Herba and its effect on isolated jejunal smooth muscle in rabbits

  • Pak J Pharm Sci. 2022 Mar;35(2):587-594.
Yu Chen 1 Weiwei Wu 2 Yingying Fang 3 Youyue Zhang 4 Heyong Zhao 5 Yuanxia Lv 3 Yunlin Liu 6 Qian Zheng 7 Xiaoqiao Wu 3 Jianwu Zhang 3
Affiliations

Affiliations

  • 1 Department of Digestive Internal Medicine, Sijing Hospital of Songjiang District, Shanghai City, China.
  • 2 Clinical Laboratory. Maternal and Child Health Care Hospital of Shandong Province, Jinan City, China.
  • 3 School of pharmacy, North Sichuan Medical College, Nanchong City, China.
  • 4 Department of clinical medicine, North Sichuan Medical College, Nanchong City.
  • 5 Department of Obstetrics. Maternal and Child Health Care Hospital of Shandong Province, Jinan City.
  • 6 Department of clinical medicine of Chinese and western medicine, North Sichuan Medical College, Nanchong City, China.
  • 7 Function Center in School of Basic Medical Science, North Sichuan Medical College, Nanchong City, China.
PMID: 35642416
Abstract

Lophatheri Herba is a traditional Chinese medicine, which is commonly used in the treatment of fever, stomatitis, urodynia. The aim of the study is to evaluate the antidiarrheal activity of the ethanol extract of Lophatheri Herba (Gramineae, ELH) and observe its effect on isolated jejunum smooth muscle in rabbits, so that we can provide a possible pharmacological basis for its clinical use. Methods: In vivo, the antidiarrheal activity of ELH (250, 500 and 1000 mg/kg; orally) in castor oil-induced Kun Ming mice was evaluated. In vitro, the effect of ELH (0.01-10 mg/mL) on the spontaneous and ACh (10μM)/K+ (60mM)-induced contraction of isolated rabbit jejunum smooth muscle was studied. The possible mechanism of spasmolytic effect of ELH (1, 3mg/mL) was explored by pretreatment of intestinal tract with CaCl2. Results: ELH (500 and 1000mg/kg) exhibited antidiarrheal effect and it (0.01-10 mg/mL) inhibited the spontaneous and ACh/K+-induced contraction with an EC50 value of 1.27 (0.89-1.34), 0.76 (0.54-1.02) and 0.34 (0.27-0.53), it also shifted the concentration-response curves of CaCl2 to right with decreased in max, similar to verapamil. ELH has significant antidiarrheal and spasmolytic effect, this provides the pharmacological basis for use in gastrointestinal disorders.

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