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  4. MeOSuc-AAPV-AMC

MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).

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MeOSuc-AAPV-AMC

MeOSuc-AAPV-AMC 構造式

CAS 番号 : 72252-90-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 116 在庫あり
Solution
10 mM * 1 mL in DMSO USD 116 在庫あり
Solid
5 mg $84 在庫あり
10 mg $120 在庫あり
25 mg $240 在庫あり
50 mg $350 在庫あり
100 mg $490 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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MeOSuc-AAPV-AMC 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm)[1].

IC50 & Target

Elastase[1]
体外実験

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].

1. Prepare The stock solutions of fluorogenic peptide substrates and stored at -80°C.
2. Dilute the stock solution～100-fold into reaction buffers to prepare 2× substrate buffers (400 μM).
3. The enzymes are then incubated for 40 min.
4. The enzymatic activity is subsequently assayed by rapidly mixing 50 μL of the substrate buffer with 50 μL of the enzyme solution in a clear bottom plates.
5. Record the fluorescence change on a fluorescence plate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MeOSuc-AAPV-AMC 関連抗体
分子量

627.69

分子式

C31H41N5O9
Emission (Em)

460
Excitation (Ex)

380
CAS 番号
Appearance

Solid

Color

White to off-white

輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

polypeptide, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (polypeptide, sealed storage, away from moisture and light)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (159.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5931 mL 7.9657 mL 15.9314 mL
5 mM 0.3186 mL 1.5931 mL 3.1863 mL
10 mM 0.1593 mL 0.7966 mL 1.5931 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5931 mL 7.9657 mL 15.9314 mL 39.8286 mL
5 mM 0.3186 mL 1.5931 mL 3.1863 mL 7.9657 mL
10 mM 0.1593 mL 0.7966 mL 1.5931 mL 3.9829 mL
15 mM 0.1062 mL 0.5310 mL 1.0621 mL 2.6552 mL
20 mM 0.0797 mL 0.3983 mL 0.7966 mL 1.9914 mL
25 mM 0.0637 mL 0.3186 mL 0.6373 mL 1.5931 mL
30 mM 0.0531 mL 0.2655 mL 0.5310 mL 1.3276 mL
40 mM 0.0398 mL 0.1991 mL 0.3983 mL 0.9957 mL
50 mM 0.0319 mL 0.1593 mL 0.3186 mL 0.7966 mL
60 mM 0.0266 mL 0.1328 mL 0.2655 mL 0.6638 mL
80 mM 0.0199 mL 0.0996 mL 0.1991 mL 0.4979 mL
100 mM 0.0159 mL 0.0797 mL 0.1593 mL 0.3983 mL
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MeOSuc-AAPV-AMC Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MeOSuc-AAPV-AMC72252-90-5Fluorescent DyeLeukocyteporcinepancreatic elastasefluorogenicsubstrateInhibitorinhibitorinhibit

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製品名:
MeOSuc-AAPV-AMC
製品番号:
HY-136865
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