1. Recombinant Proteins
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens Biotinylated Proteins
  3. TNF Superfamily Stimulatory immune checkpoint molecules Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. TNF Superfamily Ligands 4-1BBL/CD137L
  5. 4-1BBL
  6. 4-1BBL/TNFSF9 Protein, Human (Biotinylated, HEK293, Fc)

4-1BBL/TNFSF9 Protein, Human (Biotinylated, HEK293, Fc)

Cat. No.: HY-P78069
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
100 μg Ask For Quote & Lead Time

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Immobilized Human 4-1BB hFc at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer) hFc with the EC50 of 61 ng/mL determined by ELISA.
Species

Human

Source

HEK293

Tag

N-hFc

Accession

P41273 (R71-E254)

Gene ID
Synonyms
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Molecular Weight

Approximately 84.3 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
4-1BBL/TNFSF9 Protein, Human (Biotinylated, HEK293, Fc)
Cat. No.:
HY-P78069
Quantity:
MCE Japan Authorized Agent: