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  3. Erythropoietin/EPO Protein, Mouse (HEK293, His)

Erythropoietin/EPO Protein, Mouse (HEK293, His)

Cat. No.: HY-P70409
COA Handling Instructions

Erythropoietin (EPO) is essential for erythrocyte regulation, influencing proliferation, differentiation, and maintaining a balanced erythrocyte mass. Binding to its receptor, EPOR, induces dimerization and activates JAK2, initiating signaling pathways, including STAT1 and STAT3. These events are pivotal in orchestrating erythropoiesis and maintaining red blood cell homeostasis. Erythropoietin/EPO Protein, Mouse (HEK293, His) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Erythropoietin/EPO Protein, Mouse (HEK293, His) is 166 a.a., with molecular weight of 30-40 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Erythropoietin (EPO) is essential for erythrocyte regulation, influencing proliferation, differentiation, and maintaining a balanced erythrocyte mass. Binding to its receptor, EPOR, induces dimerization and activates JAK2, initiating signaling pathways, including STAT1 and STAT3. These events are pivotal in orchestrating erythropoiesis and maintaining red blood cell homeostasis. Erythropoietin/EPO Protein, Mouse (HEK293, His) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Erythropoietin/EPO Protein, Mouse (HEK293, His) is 166 a.a., with molecular weight of 30-40 kDa.

Background

Erythropoietin (EPO) is a hormone crucial for regulating the proliferation and differentiation of erythrocytes, as well as maintaining a balanced circulating erythrocyte mass. Upon binding to its receptor, EPOR, EPO triggers EPOR dimerization and subsequent activation of JAK2, initiating specific downstream signaling pathways, notably involving STAT1 and STAT3. These molecular events play a pivotal role in orchestrating the processes that contribute to erythropoiesis and the overall homeostasis of red blood cell levels.

Biological Activity

The cell proliferation assay using TF 1 human erythroleukemic cells has an ED50 value of 0.05-0.35 ng/mL.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

Q0VED9 (A27-R192)

Gene ID

13856  [NCBI]

Synonyms
rMuErythropoietin/EPO, His; Erythropoietin; Epoetin; EPO
AA Sequence

APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Molecular Weight

30-40 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Erythropoietin/EPO Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Erythropoietin/EPO Protein, Mouse (HEK293, His)
Cat. No.:
HY-P70409
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