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  5. Glycoprotein 130 (gp130)
  6. gp130/IL6ST Protein, Human (HEK293, His-Fc)

gp130/IL6ST Protein, Human (HEK293, His-Fc)

Cat. No.: HY-P75795
COA Handling Instructions

Glycoprotein 130 (gp130) is a trans-membrane protein, is the common signal-transducing receptor subunit of the interleukin-6 (IL-6) family. gp130 inhibits the cell growth and migration, decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67. gp130 shows anti-tumor, anti-inflammation and anti-nociceptive activity. gp130/IL6ST Protein, Human (HEK293, His-Fc) is a recombinant protein with a His-Fc label that consists of 618 amino acids (M1-I618) and is produced by HEK293 cells.

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Description

Glycoprotein 130 (gp130) is a trans-membrane protein, is the common signal-transducing receptor subunit of the interleukin-6 (IL-6) family[1]. gp130 inhibits the cell growth and migration[3], decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67. gp130 shows anti-tumor, anti-inflammation and anti-nociceptive activity[4][5]. gp130/IL6ST Protein, Human (HEK293, His-Fc) is a recombinant protein with a His-Fc label that consists of 618 amino acids (M1-I618) and is produced by HEK293 cells.

Background

Glycoprotein 130 mRNA is ubiquitously expressed in the human body,exhibiting highest levels in saphenous vein, pericardium, ovary, omental adipose tissue, peritoneum, spleen lymphnodes, and trigeminal ganglia. And gp130 expression is significantly increased in adult organs compared to fetal organs. Moreover, gp130 is highly expressed in multiple types of cancers compared to normal tissues[3].
The amino acid sequence of human gp130 protein has low homology between mouse and rat gp130 protein.
gp130 signaling is initiated when autocrine or paracrine cyto-kines induce the dimerization of gp130 and an α-receptor subunit to form the receptor complex, leading to185the activation of downstream signaling cascades, such as the JAK/STAT, the PI3K/Akt, and the Ras/Raf/Mek/Erk1/2MAPK pathways. The activation of JAK/Stat3 pathway induces its target gene expression, resulting in cell growth and proliferation (cyclin D, CDC25 A, c-Myc, survivin), survival (survivin, Bcl-2, Bcl-XL), angiogenesis (VEGF), and cell migration (MMP-2, MMP-7, and MMP-9)[3]. gp130 is a IL-6 antagonist shows anti-tumor, anti-inflammation and antinociceptive activity[4]. gp130 decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67[5].

In Vitro

gp13 (human) (-1.14 µg/mL; 24 h) inhibits the cell growth in a dose-dependent manner and induces apoptosis in HepG2 cells[5].
Glycoprotein 13 (human) (-2 ng/mL; 48 h) significantly decreases the STAT3 phosphorylation induced by IL-6, and decreases IL-6-induced increase of Ki67 in HepG2 cells[5].

In Vivo

Glycoprotein 13 (human) (5 µg/mL; i.p., 2 µL; every third day for 12 days) shows antinociceptive effect in antigen-induced arthritis rats[4].
Glycoprotein 13 (human) (2 µg/mL; i.a.; 5 µL; every third day for 12 days) shows antinociceptive effect in antigen-induced arthritis rats[4].
Glycoprotein 13 (human) (1.14 µg/kg; i.v.; every 3 days for 35 days) suppresses tumor growth in NOD/SCID mice with HepG2 cells injection[5].

Biological Activity

Immobilized Human gp130, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha that produces 50% of the optimal binding response is found to be approximately 6.8 ng/mL.

Species

Human

Source

HEK293

Tag

C-hFc;C-His

Accession

P40189 (E23-I618)

Gene ID
Molecular Construction
N-term
gp130 (E23-I618)
Accession # P40189
hFc-His
C-term
Synonyms
Interleukin-6 receptor subunit beta; IL-6RB; CDw130; gp130; CD130; IL6ST
AA Sequence

ELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEI

Molecular Weight

125-140 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
gp130/IL6ST Protein, Human (HEK293, His-Fc)
Cat. No.:
HY-P75795
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