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  5. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9)

IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9)

Cat. No.: HY-P70421
COA Handling Instructions

IL-23 alpha is a key component that binds to IL12B to produce IL-23 interleukin, a heterodimeric cytokine critical in innate and adaptive immunity. IL-23 functions together with IL-17 in peripheral tissues to respond acutely to infection. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9) is a recombinant protein dimer complex containing mouse-derived IL-23 alpha, expressed by Sf9 insect cells , with tag free. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9), has molecular weight of 40 & 20 kDa, respectively.

For research use only. We do not sell to patients.

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Description

IL-23 alpha is a key component that binds to IL12B to produce IL-23 interleukin, a heterodimeric cytokine critical in innate and adaptive immunity. IL-23 functions together with IL-17 in peripheral tissues to respond acutely to infection. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9) is a recombinant protein dimer complex containing mouse-derived IL-23 alpha, expressed by Sf9 insect cells , with tag free. IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9), has molecular weight of 40 & 20 kDa, respectively.

Background

IL-23 alpha, as a crucial component, associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine playing key roles in both innate and adaptive immunity. Operating in peripheral tissues, IL-23, in conjunction with IL-17, constitutes an acute response to infections. It binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activating the Jak-Stat signaling cascade, preferentially stimulating memory T-cells, and fostering the production of pro-inflammatory cytokines. This cytokine induces autoimmune inflammation, potentially contributing to autoimmune inflammatory diseases and playing a significant role in tumorigenesis. Released by antigen-presenting cells such as dendritic cells or macrophages, IL-23 binds to its receptor complex, initiating signaling pathways that activate various cellular responses, including the production of interleukin-17A/IL17A and promoting early and effective intracellular bacterial clearance. Additionally, IL-23 supports the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset known for producing IL-17, along with other IL-17-producing cells.

Biological Activity

1.Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 125.44 ng/mL. 2.Immobilized Mouse IL-23 Rα & IL-12 β at 2 μg/mL (100 μL/well) can bind IL-12R β1(HY-P70647). The ED50 for this effect is 114.8 ng/mL.

Species

Mouse

Source

Sf9 insect cells

Tag

Tag Free

Accession

Q9EQ14 (V22-A196)&P43432 (M23-S335 )

Gene ID
Molecular Construction
N-term
IL23A (V22-A196)
Accession # Q9EQ14
C-term
N-term
IL12B (M23-S335 )
Accession # P43432
C-term
Synonyms
SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2
AA Sequence

VPRSSSPDWAQCQQLSRNLCMLAWNAHAPAGHMNLLREEEDEETKNNVPRIQCEDGCDPQGLKDNSQFCLQRIRQGLAFYKHLLDSDIFKGEPALLPDSPMEQLHTSLLGLSQLLQPEDHPRETQQMPSLSSSQQWQRPLLRSKILRSLQAFLAIAARVFAHGAATLTEPLVPTA&MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Molecular Weight

42.3&20 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-23 alpha (175a.a) & IL-12 beta (313a.a) Heterodimer Protein, Mouse (Sf9)
Cat. No.:
HY-P70421
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