IL-23R Protein, Human (353a.a, HEK293, Fc)

1. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/mL (100 μl/well) can bind human IL23R-Fc and the EC₅₀ is 0.28-0.66 μg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/mL (100 μl/well) can bind human IL23R-Fc. The EC₅₀ of human IL23R-Fc is 0.14-0.35 μg/mL.
3.Measured in a cell proliferation assay using M-NFS-60 cells. The ED₅₀ this effect is 0.9517 μg/mL, corresponding to a specific activity is 1.05×10³ units/mg in the presence of 0.125 ng/mL of recombinant human G-CSF.
4. Measured by its ability to inhibit IL-23 induced IL-17 secretion by Jurkat cells. The ED₅₀ for this effect is 0.03289 μg/mL in the presence of 1 ng/mL IL-23, corresponding to a specific activity is 3.04×10⁴ U/mg.

Human

HEK293

C-hFc

AAM44229.1 (G24-D353)

149233  [NCBI]

GITNINCSGHIWVEPATIFKMGMNISIYCQAAIKNCQPRKLHFYKNGIKERFQITRINKTTARLWYKNFLEPHASMYCTAECPKHFQETLICGKDISSGYPPDIPDEVTCVIYEYSGNMTCTWNAGKLTYIDTKYVVHVKSLETEEEQQYLTSSYINISTDSLQGGKKYLVWVQAANALGMEESKQLQIHLDDIVIPSAAVISRAETINATVPKTIIYWDSQTTIEKVSCEMRYKATTNQTWNVKEFDTNFTYVQQSEFYLEPNIKYVFQVRCQETGKRYWQPWSSLFFHKTPETVPQVTSKAFQHDTWNSGLTVASISTGHLTSDNRGD

Approximately 88.18 kDa

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  Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.