1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Receptor Tyrosine Kinases
  4. INSR/CD220 Insulin Receptor (INSR)
  5. Insulin R/CD220 Protein, Human (sf9, His-GST)

Insulin R/CD220 Protein, Human (sf9, His-GST)

Cat. No.: HY-P73244
COA Handling Instructions

The insulin R/CD220 protein is a receptor tyrosine kinase that coordinates insulin action by phosphorylating substrates such as IRS and SHC upon insulin binding. Phosphorylated IRS activates PI3K-AKT/PKB to exert metabolic effects, and activates Ras-MAPK to promote growth and differentiation. Insulin R/CD220 Protein, Human (sf9, His-GST) is the recombinant human-derived Insulin R/CD220 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Insulin R/CD220 Protein, Human (sf9, His-GST) is 394 a.a., with molecular weight of ~70 kDa.

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Description

The insulin R/CD220 protein is a receptor tyrosine kinase that coordinates insulin action by phosphorylating substrates such as IRS and SHC upon insulin binding. Phosphorylated IRS activates PI3K-AKT/PKB to exert metabolic effects, and activates Ras-MAPK to promote growth and differentiation. Insulin R/CD220 Protein, Human (sf9, His-GST) is the recombinant human-derived Insulin R/CD220 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Insulin R/CD220 Protein, Human (sf9, His-GST) is 394 a.a., with molecular weight of ~70 kDa.

Background

The Insulin R/CD220 protein, a receptor tyrosine kinase, orchestrates the diverse actions of insulin through the phosphorylation of multiple intracellular substrates upon insulin binding. These substrates include insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL, and other signaling intermediates. Phosphorylated IRS proteins activate two main signaling pathways: the PI3K-AKT/PKB pathway, pivotal for insulin's metabolic effects, and the Ras-MAPK pathway, which collaborates with PI3K to regulate cell growth and differentiation. The PI3K-AKT/PKB pathway triggers the translocation of the glucose transporter SLC2A4/GLUT4 to the cell membrane, facilitating glucose transport. Activated AKT/PKB, a downstream effector, induces an anti-apoptotic effect by phosphorylating BAD, regulates the expression of metabolic enzymes, and modulates the mTORC1 signaling pathway, integrating insulin signals for cell growth and metabolism. The Ras/RAF/MAP2K/MAPK pathway mediates insulin-induced cell growth, survival, and cellular differentiation. In addition to insulin, the receptor can bind insulin-like growth factors (IGFI and IGFII). Hybrid receptors composed of IGF1R and INSR isoforms exhibit varying affinities for IGFs and insulin, influencing their activation patterns. Furthermore, in adipocytes, the receptor inhibits lipolysis.

Biological Activity

The specific activity was determined to be >45 nmol/min/mg using Poly (Ala,Glu,Lys,Tyr) 6:2:5:1 as substrate.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P06213 (G989-S1382)

Gene ID
Molecular Construction
N-term
His-GST
INSR (G989-S1382)
Accession # P06213
C-term
Synonyms
CD 220; HHF5; INSR; Insulin R; Insulin receptor; IR
Molecular Weight

Approximately 70 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4, 20% gly, 0.3 mM DTT

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Insulin R/CD220 Protein, Human (sf9, His-GST)
Cat. No.:
HY-P73244
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