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  5. Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi)

Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi)

Cat. No.: HY-P72411
COA Handling Instructions

Siglec-10 Protein, identified as a putative adhesion molecule, mediates sialic-acid dependent cellular binding, preferring alpha-2,3- or alpha-2,6-linked sialic acid. Its sialic acid recognition site may undergo masking through cis interactions. In immune responses, Siglec-10 acts as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation, recruiting SH2 domain-containing phosphatases, and negatively regulating B-cell antigen receptor signaling via PTPN6/SHP-1. In collaboration with CD24, it selectively suppresses the immune response to DAMPs, regulates NK cell response, controls autoimmunity, and inhibits cross-presentation during adaptive immune responses by CD8-alpha(+) dendritic cells. Siglec-10 interacts with proteins including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11. Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 530 a.a., with molecular weight of 100-130 kDa.

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Description

Siglec-10 Protein, identified as a putative adhesion molecule, mediates sialic-acid dependent cellular binding, preferring alpha-2,3- or alpha-2,6-linked sialic acid. Its sialic acid recognition site may undergo masking through cis interactions. In immune responses, Siglec-10 acts as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation, recruiting SH2 domain-containing phosphatases, and negatively regulating B-cell antigen receptor signaling via PTPN6/SHP-1. In collaboration with CD24, it selectively suppresses the immune response to DAMPs, regulates NK cell response, controls autoimmunity, and inhibits cross-presentation during adaptive immune responses by CD8-alpha(+) dendritic cells. Siglec-10 interacts with proteins including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11. Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is 530 a.a., with molecular weight of 100-130 kDa.

Background

Siglec-10 protein, recognized as a putative adhesion molecule, functions in sialic-acid dependent cellular binding, displaying a preference for alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site of Siglec-10 may undergo masking due to cis interactions with sialic acids on the same cell surface. In immune responses, it appears to act as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation and recruiting cytoplasmic phosphatases via their SH2 domains, blocking signal transduction through dephosphorylation of signaling molecules. Siglec-10 is involved in the negative regulation of B-cell antigen receptor signaling, dependent on PTPN6/SHP-1. In association with CD24, it may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Siglec-10, in collaboration with CD24, regulates the immune response of natural killer (NK) cells and plays a role in the control of autoimmunity. During the initiation of adaptive immune responses by CD8-alpha(+) dendritic cells, Siglec-10 inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes, implicating the recruitment of PTPN6/SHP-1 and promoting phagosomal acidification. Siglec-10 interacts with various proteins, including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11.

Biological Activity

Measured by its binding ability in a functional ELISA. 1 μg/mL (100 μL/well) of immobilized Anti-Human Siglec10 mAb can bind Siglec-10, Human (HEK 293, Fc-Avi) with an ED50 value of ≤ 1.45 ng/mL.

Species

Human

Source

HEK293

Tag

C-Avi;C-hFc

Accession

Q96LC7-1 (M17-T546)

Gene ID
Molecular Construction
N-term
Siglec-10 (M17-T546)
Accession # Q96LC7-1
hFc-Avi
C-term
Synonyms
MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; siglec-like gene 2
AA Sequence

MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLIST

Molecular Weight

100-130 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi)
Cat. No.:
HY-P72411
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