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  3. Fluorescent Dye Bacterial
  4. Safranin

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. Safranin can readily intercalate into biological macromolecules, including DNA and proteins. Safranin has antibacterial effects against gram-positive bacteria (S. aureus). Safranin can be used as a redox indicator in the determination of metal ion concentration.

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Safranin

Safranin 화학구조

CAS No. : 477-73-6

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
50 g 해외재고보유
100 g 해외재고보유
500 g 해외재고보유
> 100 g   견적 받기  

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Safranin Related Antibodies

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제품 설명

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. Safranin can readily intercalate into biological macromolecules, including DNA and proteins. Safranin has antibacterial effects against gram-positive bacteria (S. aureus). Safranin can be used as a redox indicator in the determination of metal ion concentration[1][2][3][4].

In Vitro

Safranin (0.1% (w/v), 1 min) differentially stains the cell cycle of plant cells[2].
Safranin (up to 2 μM) inhibits Complex I-driven OXPHOS (in mouse brain homogenates) capacity[3].
Safranin (0.025%) shows antibacterial activity against gram-positive bacteria (S. aureus)[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Safranin Related Antibodies
분자량

350.85

화학식

C20H19ClN4
CAS No.
Appearance

Solid

Color

Purple to black

SMILES

CC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]
선적

Room temperature in continental US; may vary elsewhere.
보관

RT, sealed storage, away from moisture and light

In solvent -80°C 1 year
-20°C 6 months
용액&용해도
In Vitro: 

DMSO : 25 mg/mL (71.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8502 mL 14.2511 mL 28.5022 mL
5 mM 0.5700 mL 2.8502 mL 5.7004 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • 몰농도 계산기

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서

SDS (476 KB)

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8502 mL 14.2511 mL 28.5022 mL 71.2555 mL
5 mM 0.5700 mL 2.8502 mL 5.7004 mL 14.2511 mL
10 mM 0.2850 mL 1.4251 mL 2.8502 mL 7.1256 mL
15 mM 0.1900 mL 0.9501 mL 1.9001 mL 4.7504 mL
20 mM 0.1425 mL 0.7126 mL 1.4251 mL 3.5628 mL
25 mM 0.1140 mL 0.5700 mL 1.1401 mL 2.8502 mL
30 mM 0.0950 mL 0.4750 mL 0.9501 mL 2.3752 mL
40 mM 0.0713 mL 0.3563 mL 0.7126 mL 1.7814 mL
50 mM 0.0570 mL 0.2850 mL 0.5700 mL 1.4251 mL
60 mM 0.0475 mL 0.2375 mL 0.4750 mL 1.1876 mL
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Safranin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Safranin477-73-6Safranine TFluorescent DyeBacterialphenazinium dye spectroscopic proberedox indicatorInhibitorinhibitorinhibit

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상품명:
Safranin
Cat. No.:
HY-D0215
수량:
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