Sialidase (α2-3-6-8-9) 

Sialidase (α2-3-6-8-9) is a broadly specific sialidase that cuts linear and branched non-reducing terminal sialic acid residues from glycoproteins, glycopeptides, and oligosaccharides. Sialidase (α2-3-6-8-9) can be used for in vitro and in vivo polysaccharide analysis and characterization as well as complete glycoprotein remodeling^[1].

Product Information
This product is expressed by recombinant Escherichia coli. The enzyme can hydrolyze sialic acid connected by α2-3, α2-6, α2-8 and α2-9 bonds on glycoproteins and oligosaccharides. The rate of hydrolyzing α2-3 and α2-6 bonds is greater than that of α2-8 and α2-9 bonds.
Storage buffer: 20 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 8.0

Instructions
1. According to the reaction system, add substrate (2 μg glycoprotein or 200 nM oligosaccharide), 1.0 μL of sialidase (α2-3,6,8,9), 2.5 μL of reaction buffer (50 mM CaCl₂, 500 mM sodium acetate, pH 5.5), and add water to 25 μL;
2. After mixing, incubate the sample at 37°C for 5-60 min. Heat at 65°C for 10 min to terminate the reaction.

Notes
The pH of the above-mentioned sialidase reaction buffer is 5.5. If you want to enzymatically cleave sialic acid on the cell surface, please replace the buffer system that is compatible with the cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Colorless to light yellow

[Sialidase (a2-3-6-8-9)]

≥50 U/μL

One unit is defined as the amount of enzyme that will cut >95% of terminal α-Neu5Ac from 1 nmol Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC) in a total reaction volume of 10 μL at 37℃ within 5 min.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Means RE, Desrosiers RC. Resistance of native, oligomeric envelope on simian immunodeficiency virus to digestion by glycosidases. J Virol. 2000 Dec;74(23):11181-90.  [Content Brief]